專技 - 藥物分析與生藥學(包括中藥學)題庫下載題庫

(B) Borate gels  課本敘述 
1.  immobilised alkyl boronic acids.
2.  selective affinity for 1,2- or 1,3-diol groupings 
    ( catechol-containing molecules=dopamine、sugars、glycosides)
3. The analyte is loaded onto the gel in a buffer at ca pH 7.0 
4. The complex formed can  be broken down using a mildly acidic eluent such as 0.1 M acetic acid.
5.  Applied to the determination of dopamine, adrenaline and noradrenaline in plasma and to the determination of the extent of reaction of glucose with serum albumin as a measure of glucose fluctuations with time in diabetics

33...

(B) Borate gels  課本敘述 
1.  immobilised alkyl boronic acids.
2.  selective affinity for 1,2- or 1,3-diol groupings 
    ( catechol-containing molecules=dopamine、sugars、glycosides)
3. The analyte is loaded onto the gel in a buffer at ca pH 7.0 
4. The complex formed can  be broken down using a mildly acidic eluent such as 0.1 M acetic acid.
5.  Applied to the determination of dopamine, adrenaline and noradrenaline in plasma and to the determination of the extent of reaction of glucose with serum albumin as a measure of glucose fluctuations with time in diabetics

33.以層析法進行糖化血色素（HbA1c）分析測定時，選用何種固定相較適宜？
(A)ODS
(B)Borate gel
(C)Silica gel
(D)Alumina .

課本是講糖化白蛋白 Glycosylated Albumin
所以類推糖化血色素 Glycosylated Hemoglobin

重點是糖 的 diol groups

Solid Phase Extraction SPE 固相萃取法

利用Van der Waals滯留非極性藥物

例子：固醇類

弱酸性藥物（Methicillin）以陰離子萃取後，再以緩衝液沖提

易被取代的沖提離子：OH^-、C₂H₅COO^-、CH₃COO^-、F^-

表面修飾成aminopropyl / diol的形式

滯留極性基團

弱鹼性藥物（Adrenaline）以陽離子萃取後，再以緩衝液沖提

易被取代的沖提離子：K⁺、Na⁺、NH₄⁺

滯留極性基團

沖提液：Ethanol/Methylene chloride

能與Dopamine的catechol基團、醣類、配糖體錯合

可用於血液中的Adrenaline、也可測糖尿病患者葡萄糖/白蛋白的反應程...

Solid Phase Extraction SPE 固相萃取法