5. The hyperchromic effect observed when DNA is denatured refers to: (A) The decrease in absorbance at 260 nm upon separation of strands. (B) The increase in absorbance at 260 nm when strands separate. (C) The increase in absorbance at 260 nm when strands are degraded. (D) The shift in absorbance peak to 280 nm after hydrolysis. (E) The shift in absorbance peak to 260 nm after hydrolysis.