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90. A researcher purified a specific protein and performed structural analysis using two different techniques. The data are as follows: (1) Size-Exclusion Chromatography: Under native (non-denaturing) conditions, the protein elutes at a position corresponding to a molecular weight of 200 kDa; (2) SDS-PAGE (with β-mercaptoethanol): After boiling the sample in SDS and a reducing agent, a single sharp band appears at 50 kDa. Which of the following best describes the native structure of this protein? (A) A monomeric 200 kDa protein that is non-specifically degraded into 50 kDa fragments during electrophoresis (B) A homotetramer composed of four 50 kDa subunits held together by non-covalent interactions or disulfide bonds (C) A homodimer composed of two 100 kDa subunits that were incorrectly measured during chromatography (D) A heterotetramer composed of two 75 kDa subunits and two 25 kDa subunits. (E) A 200 kDa protein that consists of a single polypeptide chain containing four internal protease cleavage sites

89. The figure below shows the absorbance curve at 260 nm of five 100 bp double-stranded DNA fragments (samples A to E) when subjected to heat. DNA denaturation produces a hyperchromic effect. Which sample has the highest G-C content, according to this graph? (A) A (B) B (C) C (D) D (E) E

88. Glucose-6-phosphatase is a key regulatory enzyme localized in the endoplasmic reticulum membrane. In von Gierke disease, the deficiency of this enzyme prevents the hydrolysis of glucose-6-phosphate. Which of the following biochemical pathways is/are directly impaired by this deficiency during fasting? (A) Glycogenesis only (B) Glycogenolysis only (C) Gluconeogenesis only (D) Both glycogenolysis and gluconeogenesis (E) The pentose phosphate pathway

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8 犯槍砲彈藥刀械管制條例之罪自首,並報繳其持有之全部槍砲、彈藥、刀械者,如於內政部報經行政院核定辦理公告期間,其減免之規定如何? (A)減輕其刑至二分之一(B)減輕其刑至三分之二(C)減輕或免除其刑(D)免除其刑

38. 液壓式噴油閥的啟閥壓力決定於下列何者? (A) 噴油泵供油的油壓 (B) 噴油閥內的油壓 (C) 噴油閥針閥彈簧的預緊力 (D) 高壓油管的油壓

11. 下列那一項作業,不屬於「放射性物質與可發生游離輻射設備及其輻射作業管理辦法」 所列放射性物質、可發生游離輻射設備或其使用場所之改裝? (A)變更輻射防護屏蔽 (B)變更主射束方向 (C)增加設備之電壓 (D)變更曝露時間

5. 孫老師對正在拍球的小蓮說:「怎樣才能夠讓你的球跳得更高?」他的問題最可以引發小 蓮下列哪種認知能力? (A) 變通力 (B) 精進力 (C) 挑戰力 (D) 冒險力

10 高透氧硬式隱形眼鏡試片度數-3.50 DS/基弧 7.90 mm/鏡片光學區 8.0 mm/鏡片直徑 9.4 mm,戴鏡驗光(over- refraction)最佳視力為 1.0 之度數是+5.00DS/-0.5DC×180,頂點距離為 12 mm。若配鏡處方基弧欲修正為 7.80 mm,則此隱形眼鏡的屈光度數應訂為多少? (A)+1.00 DS (B)+1.50 DS (C)+2.00 DS (D)+2.50 DS

8. IALA基點標誌中,關於北方基點的顏色,下列敘述何者正確?(A) 上黑下黃(B) 上黃下黑(C) 黑黃橫條紋,中為黃,上下為黑(D) 黑黃橫條紋,中為黑,上下為黃