101年 - 101 國立嘉義大學_碩士班招生考試試題_生化科技學系：分子生物學#146281

1. During (A) the centrosomes move to opposite sides of the nucleus, and the chromosomes condense. The nucleus breaks down, and as the cell progresses to (B) , the chromosomes align on the center of the spindle. At (C) the linkage between sister chromatids is broken, and the sisters segregate to opposite poles. As the chromosomes near the poles, the physical process of cell division, called (D) . (8%)

2. Please explain how the cell condense the chromosome during mitosis? (4%)

3. Please describe how the sister chromatids separation at anaphase? (5%)

4. Please describe the molecular mechanism of DNA replication once in S phase. (5%)

5. Please explain the molecular mechanism of positive feedback loop for G1 cyclin expression in mammalian cells. (3%)

1. Activation of the expression of lac operon consists of two distinct phases: (i) Basal level of expression, and (ii) Further stimulation. What this refers to? Briefly describe it. (6%)

2. Repression of trp operon also consists of two distinct mechanisms. Briefly describe them. (6%)

3. In the lytic phase of  phage infection, which gene product is responsible for the switch to the transcription beyond the delayed early genes. (3%)

4. An efficient N-mediated antitermination involves 5 proteins. Briefly describe their roles the N-mediated antitermination. (10%)

1. The X rays produce a mutation, discuss the consequences of a germ-line versus a somatic cell mutation. (10%)

2. Distinguish between spontaneous and induced mutations. Which are more harmful? Which are avoidable? (10%)

3. Most forms of cancer are caused by environment agents that produce mutations in somatic cells. Is an individual with cancer considered a genetic mosaic? Explain why or why not. (5%)

1. “Competitor” reverse transcriptase (RT)-PCR. (5%)

2. Nested PCR. (5%)

3. Inverse PCR (IPCR). (5%)

4. Random amplified polymorphic DNA (RAPD)-PCR. (5%)

5. Cleaved amplified polymorphic sequence analysis (CAPS). (5%)