108年 - 108 長庚大學_研究所碩士班招生考試_生物醫學研究所碩士班：生物化學#125899

1. If we have one DNA sequence with a 5-minutes PCR cycle time. After 1 hour, how many DNA sequence can we get? (A) 1024 (B) 2048 (C) 3072 (D) 4096

2. Which statements of SDS PAGE is correct? (A) SDS acts as a detergent and attaches to the protein, so the protein has positive charge. (B) Direction of protein movement is from positive charge (+) to negative charge (-). (C) APS (ammonium persulfate) as a buffer of SDS PAGE.(D) High molecular weight protein would stay in the upper section of the SDS PAGE.

3.   A   is a common experimental method to detect protein in a biological sample.   B   can investigate DNA in the biological sample. Dr. Kary Banks Mullis builts a method   C   which can ampilify DNA in a short period of time. Based on these description, what are methods   A  ,   B   and   C  ? (A) A: Southern blot, B: Eastern blot, C: CPR. (B) A: Southern blot, B: Western blot, C: CPR. (C) A: Western blot, B: Northern blot, C: PCR. (D) A: Western blot, B: Southern blot, C: PCR.

4. Which statement is correct? (A) Nucleoside contains nitrogen base, pentose sugar and phosphate group (B) Watson and Crick discovered RNA is a double helix structure (C) Gout patients should decrease ingestion of high purine food to release symptom (D) DNA is composed of A, T, G, C. A is a kind of pyrimidine.

5. 2D-PAGE is a method to investigate protein. Differential migration of analytes in 2D-PAGE let us measure protein expression difference between two samples. The rate of migration does not depend on? (A) Isoelectric point (B) Fluorescence (C) Protein size (D) Molecular weight

6. Which two amino acids can bind together and form disulfide bond? (A) Methionine and cysteine. (B) Serine and threonine. (C) Lysine and tyrosine. (D) Cysteine and cysteine.

7. Which one is the lowest molecular weight carbohydrate? (A) Cellulose (B) Glyceraldehyde (C) Glucose (D) Xylose

8. The effect of the deletion of one copy of the gene encoding p53 is different from the effects caused by other p53 mutations. For example, some loss-of-function mutations in the DNA-binding domain of p53 cripple its function as a transcription regulator. Such a mutation in only one copy of the p53 gene can be enough to confer a p53 loss-of-function phenotype, even when the other copy of the gene on the homologous chromosome is wild type. This is because ... (A) p53 is a proto-oncogene. (B) these mutations are recessive. (C) p53 is a tumor suppressor. (D) p53 forms a tetramer.

9. Mutations in two important cancer-critical genes, encoding p53 and Rb, respectively, are commonly found in cancers. What type of mutations are these expected to be? (A) Loss-of-function mutations in both genes (B) Loss-of-function mutation in p53 and gain-of-function mutation in Rb (C) Gain-of-function mutation in p53 and loss-of-function mutation in Rb

10. Which enzyme of the inosinic acid biosynthesis pathway is the major control point for purine synthesis? (A) phosphoribosyl pyrophosphate amidotransferase (B) glycinamide ribonucleotide transformylase (C) formylglycinamidine ribonucleotide amidotransferase (D) 5-aminoimidazole ribonucleotide synthetase

11. What molecule contributes at least two nitrogen atoms to each purine? (A) asparagine (B) 10-formyltetrahydrofolate (C) glycine (D) glutamine

12. Which of the following types of DNA repair is often coupled with transcription? (A) photoreactivation (B) base excision repair (C) replication (D) nucleotide excision repair

13. Which of the following urea cycle intermediates acts as a sensor for the regulation of this cycle at the earliest step? (A) ornithine (B) citrulline (C) argininosuccinate (D) arginine

14. A patient with glucose-6-phosphatate deficiency may have a gouty symptom because of the increased_____ flux. (A) glycolysis (B) gluconeogenesis (C) pentose phosphate pathway (D) urea cycle

15. During purification of an antibody with a protein G affinity column, 0.1 M acetic acid was used as the elution buffer. After elution, which of the following buffers (equal volume) is most suitable for bringing the pH value of the eluate to ~8.0? (Tris has a pKa of 8.1) (A) 0.2 M Tris base (B) 0.2 M Tris-HCl (C) 0.2 M Tris, pH 8.0 (D) 0.1 M NaOH

16. One-carbon transfer reaction is important in the metabolism of nitrogen-containing biomolecules. Which of the following reactions provides the major source of the required one-carbon groups? (A) serine → 5,10-methylene-THF (B) histidine → 5-formimino-THF (C) formate → 10-formyl-THF (D) glycine → 10-methyl-THF

17. Which of the following anticancer/antimicrobial drugs acts through formation of covalent bonds with its target enzyme? (A) sulfanilamide (B) 5-fluorouracil (C) trimethoprime (D) 6-mercaptopurine

18. Which of the following causes denaturation of a protein when disulfide bonds are present? (A) Sucrose (B) Dithiothreitol (C) Sodium dodecyl sulfate (D) All of the above

19. Which of the following signaling systems actually moves a molecule across the plasma membrane and interacts with its receptor in the nucleus? (A) G-protein coupled receptors (B) Tyrosine kinase receptors (C) Adhesion receptors (D) Steroid hormone receptors

20. The molecular weight of protein or peptide can be determined by: (A) Enzyme-linked immunosorbent assay (B) Immunofluorescence staining (C) Ion-exchange liquid chromatography (D) Mass spectrometry

21. Which one described below is true for the protein structure? (A) Reverse turn is often found connecting two α-helix structures. (B) Disulfide bonds are most important in quaternary structure. (C) The overall folding of a single protein subunit is called tertiary structure. (D) A domain is a folded region of protein which has specific biological function and never shared by proteins.

22. (1) immunoflorescence staining (2)co-immunoprecipitation assay (3) in vitro protein binding assay (4) chromatin immunoprecipitation assay (5) polymerase chain reaction Which method can be applied to analyze the potential interaction between two proteins? (A) 1, 2, 3 (B) 2, 3, 4 (C) 3, 4, 5 (D) 1, 2, 4

23. Which one of the following statements is not true for protein unfolding or misfolding? (A) May cause the human diseases. (B) May increase the water solubility of protein. (C) May increase the protein stability. (D) May lead to protein aggregation in the cytoplasm.

24. What does amphipathic mean? (A) having both positive and negative charges (B) having both acid and base properties (C) having both hydrophilic and hydrophobic regions (D) having two stereoisomers

25. The carrier molecule which transports fatty acids through the inner mitochondrial membrane is (A) ATP. (B) Carnitine. (C) Coenzyme A. (D) Lipoic Acid.

26. Where in the cell does β-oxidation occur? (A) Mitochondrial matrix. (B) Outer mitochondrial membrane (C) Mitochondrial intermembrane space. (D) Inner mitochondrial membrane.

27. How many cycles of β-oxidation are required to convert stearic acid (18 carbons) to 9 acetyl-CoAs? (A) 6 (B) 8 (C) 9 (D) 18

28. Two samples containing identical mixtures of sugars are analyzed, one using Tollen's reagent, the other with glucose oxidase. Which will give a stronger reaction? (A) Tollen's reagent (B) glucose oxidase (C) They will have identical strengths (D) It's impossible to predict from the information provided.

29. Which enzyme is the key regulatory enzyme in glycolysis? (A) Glyceraldehyde-3-phosphate dehydrogenase (B) Enolase (C) Phosphofructokinase (D) Aldolase

30. Uncoupling of mitochondrial oxidative phosphorylation: (A) allows continued mitochondrial ATP formation, but halts O₂ consumption. (B) halts all mitochondrial metabolism. (C) halts mitochondrial ATP formation, but allows continued O₂ consumption. (D) slows down the citric acid cycle.

31. If malonyl-CoA is synthesized from and unlabeled acetyl-CoA, and the labeled malonate is then used for fatty acid synthesis, the final product (fatty acid) will have radioactive carbon in: (A) every C. (B) every even-numbered C-atom. (C) every odd-numbered C-atom. (D) no part of the molecule.

32. In the synthesis of phosphatidylcholine from phosphatidylethanolamine, the methyl group donor is: (A) a tetrahydrofolate derivative. (B) choline. (C) methanol. (D) S-adenosylmethionine (adoMet).

33. The coenzyme involved in a transaminase reaction is: (A) biotin phosphate. (B) lipoic acid. (C) nicotinamide adenine dinucleotide phosphate . (D) pyridoxal phosphate (PLP).

34. Which amino acids supply carbons for eventual entry into metabolism as succinyl CoA? (A) Met, Val, Leu (B) Met, Ile, Val (C) Ile, Val, Leu (D) All of the above

35. The starting materials necessary to synthesize sphingosine are (A) palmitoyl CoA and serine. (B) acetyl CoA and glycine. (C) palmitoyl CoA and aspartate. (D) acetyl CoA and glutamine.

(1)____ Attacks and attaches to the central carbon in pyruvate. (A) Coenzyme A (CoA-SH) (B) NAD+ (C) Thiamine pyrophosphate (TPP) (D) FAD (E) Lipoic acid in oxidized form

(2)____ Oxidizes FADH2. (A) Coenzyme A (CoA-SH) (B) NAD+ (C) Thiamine pyrophosphate (TPP) (D) FAD (E) Lipoic acid in oxidized form

(3)____ Accepts the acetyl group from reduced lipoic acid. (A) Coenzyme A (CoA-SH) (B) NAD+ (C) Thiamine pyrophosphate (TPP) (D) FAD (E) Lipoic acid in oxidized form

(4)____ Oxidizes the reduced form of lipoic acid. (A) Coenzyme A (CoA-SH) (B) NAD+ (C) Thiamine pyrophosphate (TPP) (D) FAD (E) Lipoic acid in oxidized form

(5)____ Initial electron acceptor in oxidation of pyruvate. (A) Coenzyme A (CoA-SH) (B) NAD+ (C) Thiamine pyrophosphate (TPP) (D) FAD (E) Lipoic acid in oxidized form

3. Please describe these terms:  (A) Ketone bodies (B) Fatty liver (C) DNA replication (D) Anabolism (E) Oxidation

1. Briefly describe the relationship of the pyruvate dehydrogenase complex reaction to glycolysis and the citric acid cycle.

4. Please translate the following sentence into Chinese  Biochemistry describes the structure, organization, and functions of living matter in molecular terms.