110年 - 110長庚大學_碩士班招生考試_生物醫學研究所︰生物化學#101180

複選題
1. Which one of the following protein structural motif is known to be involved in protein-protein interaction? (A) RING (Really Interesting New Gene) finger domain (B) Ankyrin repeat (C) Helix-loop-helix (D) Zinc finger

2. Which of the following techniques is primarily undertaken to detect COVID-19? (A) RT-9PCR (B) Microarrays (C) CRISPR (D) RNA-seq

3. By adding SDS (sodium dodecyI sulfate) during the electrophoresis of proteins, it is possible to: (A) determine a protein's isoelectric point. (B) dctermine an enzyme's specific activity. (C) determine the amino acid composition of the protein. (D) separate proteins exclusively on the basis of molecular weight.

4. The PCR reaction mixture does not include: (A) all four deoxynucleoside triphosphates. (B) DNA ligase. (C) heat-stable DNA polymerase. (D) oligonucleotide primers.

5. Regarding insulin in of fuel metabolism, which of the following is false? (A) Insulin signals the fed state. (B) Insulin promotes uptake of fuel substrates. (C) Insulin promotes storage of fuels. (D) Insulin promotes gluconcogenesis in liver.

6. In the citric acid cycle, acetyl-CoA reacts with ______ to produce______(A)fumarate； a-ketoglutarate (B) oxaloacetate； citrate (C) citrate； isocitrate (D) succinyl-CoA； oxaloacetate

7. Which of the following reactions is commonly used to replenish intermediates of the citric acid cycle? (A) citrate→ oxaloacetate + acetyl CoA (B) oxaloacetate→＞ aspartate (C) glutamate →＞ a-ketoglutarate (D) malate ＞ pyruvate

8. What is the source of acetyl-CoA for the glyoxylate cycle? (A) fatty acids (B) pyruvate (C) amino acids (D) cholesterol

9. During exercise, which of the following is not likely to happen in muscle cells? (A) LDH (lactate dehydrogenase) is increased and lactate fermentation takes place (B) IDH (Isocitrate dehydrogenase) is activated by ADP and TCA cycle increases (C) PDH (Pyruvate dehydrogenase) phosphatase is activated by Ca't and TCA cycle increases (D) PDH (Pyruvate dehydrogenase) kinase is activated by ATP and TCA cycle increases

10. In the electron transport chain of mammalian cells, what is the electron donor generated from TCA cycle, and the final electron acceptor? (A) NADPH, Pyruvate (B) NADPH, O₂ (C) NADH, O₂ (D) FADH₂, Cytochrome C

11. In pH 7.0, A is a nonpolar amino acid. B is a positive-charged amino acid. C is a negative-charged amino acids. What's A, B and C? (A) A: Leu, B: Arg, C: Asp (B) A: Ala, B: Tyr, C: Thr (C) A: Ser, B: Thr, C: Glu (D) A: Cys, B: Lys, C: Gln

12. Some enzymes may participate in glycolysis pathway and gluconeogenesis pathway. Which one is only participate in glycolysis pathway? (A) Hexokinase (B) Phosphoglucose isomerase (C) Aldolase (D) Phosphoglycerate mutase

13. Which one is carbohydrate with the lowest molecular weight? (A) Cellulose (B) Glyceraldehyde (C) Glucose (D) Xylose

14. Which statements about SDS PAGE is correct? (A) SDS is a kind of detergent which can attach to proteins have only positive charges. (B) Moving direction of the protein is from positive charge (+) to negative charge (-). (C) APS (ammonium persulfate) is used as the buffer of SDS PAGE. (D) High-molecular-weight protein would stay in the upper section of SDS PAGE

15. A peptide with amino acid sequence as 'N'-MNKLVAAEGYYFHTTVNAKDFFH-C' ", When this peptide is digested by trypsin. How many amino acids is in the longest peptide? (A) 14 (B) 15 (C) 16 (D) 17

16. Which amino acid may not be a precursor for the synthesis of Compound I?

(A) glutamate (B) proline (C) lysine (D) histidine

17. Which of the following vitamin deficiencies may result in hyperhomocysteinemia? (A) vitamin Bi2 (cobalamin) (B) vitamin B. (pyridoxine) (C) folic acid (D) all of the above

18. Which of the following is an essential amino acid although an enzyme for its synthesis is present in human? (A) lysine (B) arginine (C) cysteine (D) tyrosine

19. What reaction is common in the biosynthesis of neurotramsmitters such as histamine, dopamine, GABA, and serotonin? (A) transamination (B) oxidation (C) hydroxylation (D) decarboxylation

20. 3' - Thiacytidine (3TC) can serve as a drug for HIV infections. Which is the correct structure of this compound? (A)(B)(C)(D)

21. Which carbon of the open-chain form of a sugar becomes the anomeric carbon in the cyclic fomm? (A) the carbonyl carbon (B) the highest numbered chiral center (C) the highest numbered carbon (D) It depends on the individual sugar.

22. Lactose intolerance (A) arises from inability to metabolize the disaccharide lactose (B) depends on a deficiency of sucrose in the diet (C) is based on the composition of lactose, consisting glucose and fructose in glycosidic linkage (D) rises because lactose is a dimer of galactose

複選題
23. In bacterial cell walls (A) polysaccharides form nonspecific mixtures with proteins (B) polysaccharides are hydrogen bonded together (C) peptides form crosslinks between polysaccharides (D) oligosaccharides form crosslinks between proteins

24. Which enzyme cleaves the a(1 6) bonds in glycogen? (A)glycogen phosphorylase (B) debranching enzyme (C) phosphoglucomutase (D) glycogen synthase

25. Glucose, labeled with in different carbon atoms, is added to a crude extract of a tissue rich in the enzymes of the pentose phosphate pathway. The most rapid production of , will occur when the glucose is labeled in: (A) C-1 (B) C-3 (C)C-4 (D) C-5

26. A suspension of which of the following is most likely to form a liposome?
I fatty acids II. Triacylglycerols III. Glycerophospholipids V. sphingomyelins(A) I, II(B) II, III(C) III, IV(D) I, IV

27. Lung surfactants function to decrease the likelihood of collapse of the alveoli when air is expired. Based on your knowledge of lipids and amphipathic molecules, which of the following explains the mechanism by which the dipalmitoyI phosphatidylcholine (DPPC) can accomplish this task? (A) The choline portion of the molecule is bulky, thus increasing surface area of alveoli. (B) 'The inclusion of saturated fatty acids on the glycerophospholipid allows close packing of tail groups which are fully extended and prevent collapse upon expiration. (C) The hydrophilic head groups are bulky and expand the surface area of the alveoli. (D) The hydrophobic portion of the DPPC repel air, foreing the alveoli space open

28. If malonyl-CoA is synthesized from and unlabeled acetyl-CoA, and the labcled malonate is then used for fatty acid synthesis, the final product (fatty acid) will have radioactive carbon in: (A) every C. (B) every even-numbered C-atom. (C) only the omega-carbon atom (farthest carbon from C-1) (D) no part of the molecule.