110年 - 110長庚大學_碩士班招生考試_醫學生物技術暨檢驗學系︰生物化學#101169

科目：研究所、轉學考(插大)◆生物化學 | 年份：110年 | 選擇題數：25 | 申論題數：0

試卷資訊

所屬科目：研究所、轉學考(插大)◆生物化學

選擇題 (25)

1. You are trying to separate proteins A, B, C, D, and E by gel filtration chromatography: Which of the proteins will be eluted in the second order from the column? (A) protein A (42 kDa) (B) protein B (99 kDa) (C) protein C (64 kDa) (D) protein D (121 kDa) (E) protein E (82 kDa)

2. Which of the following amino-acid combinations have side chains with groups that have the greatest ability to stabilize the tertiary structure of a protein? (A) Kand R(B)CandE (C)Dand N(D) DandR(E) Wand E

3. Please select the most suitable method for separation of peptides PSVIA and ANTSP. (A) affinity chromatography (B) size-exclusion chromatography (C) ion-exchange chromatography (D)gel fitration chromatography (E) reversed-phase liquid chromatography

4. Which of the following pairs of bonds within a peptide backbone show free rotation around both bonds? (A) C=O and N-C (B) Ca-C and N-C. (C)C=Oand N-C. (D)N-Cand Co-c (E) N-Ca and N-C

5. Cooperative binding between ligands and proteins can be described quantitatively with the Hill equation. The slope in the Hill plot actually reflects (A) homotropic interaction (B) number of ligand bound (C) degree of interaction (D) affinity (E) heterotropic interaction

6. Which cofactors are involved in the conversion of pyruvate to acety-CoA? (A) Biotin, flavin adenine dinucleotide (FAD), and thiamine pyrophosphate (TPP) (B) Biotin, nicotinamide adenine dinucleotide (NAD'), and FAD (C) NAD*, biotin, and TPP (D) Pyridoxal phosphate, FAD, and lipoic acid (E) TPP, lipoic acid, and NAD

7. The two moles of COz produced in the first turn of the citric acid cycle have their origin in the (A) carboxyl and methylene carbons of oxaloacetate. (B) carboxyl group of acetate and a carboxyI group of oxaloacetate. (C) carboxyI group of acetate and the keto group of oxaloacetate. (D) two carboxyI groups derived from oxaloacetate. (E) two carbon atoms of acetate.

8. If electron transfer in tightly coupled mitochondria is blocked (with antimycin A) between cytochrome b and cytochrome ct, then (A) all ATP synthesis will stop. (B) ATP synthesis will continue, but the P/O ratio will drop to one. (C) electron transfer from NADH will cease, but Oz uptake will continue. (D) electron transfer from succinate to Oz will continue unabated. (E) energy diverted from the cytochromes will be used to make ATP, and the P/O ratio will rise.

9. During oxidative phosphorylation, the proton motive force that is generated by electron transport is used to (A) create a pore in the inner mitochondrial membrane. (B) generate the substrates (ADP and Pi) for the ATP synthase. (C) induce a conformational change in the ATP synthase. (D) oxidize NADH to NAD+. (E) reduce Oz to H2O.

10. Which of the following statements concerning the B oxidation of fatty acids is true? (A) About 1,200 ATP molecules are ultimately produced per 20-carbon fatty acid oxidized. (B) One FADH2 and two NADH are produced for each acetyl-CoA. (C) The free fatty acid must be carboxylated in the B position by a biotin-dependent reaction before the process of 8 oxidation commences. (D) The free fatty acid must be converted to a thioester before the process of B oxidation commences, (E) Two NADH are produced for each acetyl-CoA.

11. The ribosome is the key organelle for protein translation in a cell. Which of the followings is not true about ribosome? (A) The intact ribosomes in eukaryotic cells are assembled by the small (40S) and large (60S) ribosomal subunits (B) Two ribosomal subunits must dissociate after each round of translation for new initiation complex to form (C) A ribosome contains ribosomal RNAs and ribosomal proteins (D) The initial aminoacryI tRNA binds to the P site of a ribosome during protein translation (E) The peptidyl transferase activity is on the large subunit of a ribosome

12. elF4F is a complex of eukaryotic translation initiation factors that binds to the cap structure of mRNA. Which of the followings is one of the components of elF4F? (A)elF4A(B) elF2B (C) elf1 (D) elF3 (E) elF5

13. There are several kinds of RNA polymerases in eukaryotic cells. What is the RNA polymerase that makes tRNA in eukaryotic cells? (A) RNA polymerase I (B) RNA polymerase I| (C) RNA polymerase III (D) RNA polymerase IV (E) RNA polymerase V

14. Which of the followings is not a posttranscriptional RNA processing event in eukaryotic cells? (A) Phosphorylation of translational initiation factor (B) RNA splicing (C) RNA editing (D) mRNA capping (E) polyadenylation of mRNA

15. Which of the followings is one of the mechanisms that repair DNA damage in eukaryotic cells? (A) Phosphorylation repair (B) Double-strand break repair (C) Ubiquitination repair (D) Sumoylation repair (E) tRNA-mediated repair

複選題
16. Which of the following statement about isoenzymes and isoforms of enzymes is correct? (A) Different isoenzymes have the same catalytic properties for the same reaction. (B) Both isoenzymes and isoforms can have tissue-specific distribution. (C) Phosphorylation is one mechanism leading to the formation of different isoenzymes. (D) Different isoforms are encoded by different genes. (E) Different isoenzymes are encoded by the same genes.

17. Which of the following statement is correct about reversible inhibition of enzymatic reactions? (A) Km is reduced in noncompetitive inhibition. (B) A competitive inhibitor is structurally different from the substrate of the enzyme. (C) Vmax in competitive inhibition is unaffected. (D) When plotting as Lineweaver-Burk Plot, the straight line for the condition with uncompetitive inhibitor and that of the condition without an inhibitor have the same Y-axis intercept. (E) None of about is correct.

18. Which of the following statement about coenzymes and prosthetic groups is not correct? (A) coenzyme is an organic non-protein compound that binds with an enzyme to catalyze a reaction. (B) Coenzymes are bound only momentarily to the enzyme during the course of the reaction. (C) Prosthetic group binds to the enzyme to form the active holoenzyme. (D) Pyridoxal-5'-phosphate is the prosthetic group of dehydrogenases. (E) NAD*/NADH and NADP*/NADP are classified as coenzymes of the enzymes.

19. Which enzyme catalyzes the reaction NDP → dNDP? (A) thymidylate synthase (B) ribonucleotide kinase (C) hypoxanthine phophoribosyI transferase (D) topoisomerase (E) ribonucleotide reductase

20. Double stranded DNA molecules can be cleaved at specific recognition sites by (A) RNA polymerase.(B) DNA ligase. (C) DNA polymerase. (D) gyrase. (E) restriction endonucleases.

21. Which one of the following is the major component of cell membrane? (A) cholesterol (B) triacylglycerol (C) terpene (D) phospholipid (E) sphingolipid

22. GLUT 1 is a transporter for glucose uptake. Which mechanism is used by this receptor? (A) simple diffusion (B) facilitated transportation (C) activate transportation (D) act as an ionophore (E) none of above

23. The expression of phosphatidyIserine on cell surface could be observed in which cell behavior? (A) apoptosis (B) autophagy (C) phagocytosis (D) cell replication (E) pyroptosis

24. Which linkage is presented in sucrose in between? (A) a-1.8-2-glycosidic (B) B-1,4-glycosidic (C) a-1,4-glycosidic (D) B-1,6-glycosidic (E) a-1,6-glycosidic

25.Which one of the following is the substrate for cyclooxygenase-2? (A) arachidonic acid (B) triacylglycerol (C) estradiol (D) prostaglandins (E) leukotriene

申論題 (0)