複選題
15. Which enzyme(s) is/are NOT required for setting up a Real-Time quantitative reverse transcription PCR experiment?
(A) E. coli DNA polymerase I
(B) T4 DNA ligase
(C) Taq DNA polymerase
(D) Reverse transcriptase
(E) T7 RNA polymerase

11.RNA interference (RNAi) is an evolutionarily conserved mechanism to degrade RNA molecules in a sequence-specific manner. This mechanism does NOT exist in (A) Homo sapiens (B) Drosophila melanogaster (C) Caenorhabditis elegans (D) Saccharomyces cerevisiae (E) Escherichia coli

複選題12.MicroRNAs are small non-coding regulatory RNAs that down regulate gene expression by the mechanisms including (A) Repress translation of target mRNAs (B) Trigger cleavage of target mRNAs (C) Trigger decapping of target mRNAs (D) Enhance polyadenylation of target mRNAS (E) Trigger the nonsense-mediated decay (NMD) pathway

13.During the first stage of translation, the small and large ribosomal subunits assemble around an mRNA that has an aminoacylated initiator tRNA correctly positioned at the start codon. The process is mediated by a special set of proteins known as translation initiation (IFs). In eukaryotes, the translation initiation complex contains the 40S subunit, the Met-tRNA and IFs NOT including (A) eIF1 (B) eIF2 (C) eIF3 (D) eIF4 (E) eIF6

複選題14. Which of the following techniques can be used to measure the amount of a specific mRNA in a sample? (A) Real-Time quantitative reverse transcription PCR (B) Primer extension analysis (C) Northern blot analysis (D) Southern blot analysis (E) Western blot analysis

複選題16.Protein-Protein interaction can be examined by yeast two-hybrid and co-immunoprecipitation. Which methods can be used for the same purpose as well? (A) Electrophoretic mobility shift assay (EMSA) (B) Farwestern blot analysis (C) Glutathione-S-transferase (GST) fusion protein pull-down (D) Förster resonant energy transfer (FRET) (E) Bimolecular fluorescence complementation (BiFC)

複選題17. Which method(s) can be used for detecting RNA-Protein interaction? (A) Electrophoretic mobility shift assay (EMSA) (B) Real-Time quantitative reverse transcription PCR (C) Protein-RNA pull-down assays (D) Fluorescent in situ hybridization (FISH) (E) Oligonucleotide-targeted RNase H protection assays

18. Which method(s) can be used to functionally inactivate a gene without changing its DNA sequence? (A) CRISPR/Cas9 genome editing (B) RNAi interference (C) Site directed mutagenesis (D) TALEN genome edditing (E) Cre-Lox and FLP/FRT recombinase target-based gene inactivation

複選題19. Which consensus sequences will define a typical intron? (A) 5' splice site (B) 3' splice site (C) Branch points (BPs) (D) Polypyrimidine tract (E) Kozak consensus sequence

複選題20. Which of the following evidence will indicate a discovery of a gene in a DNA sequence? (A) The sequence is aligned partially to a known cDNA sequence (B) The sequence is similar to a known genes in other organisms (C) The sequence is AT-rich (D) Typical intron splice sites are found (E) Typical poly( A ) sites are found

21.Please select the CORRECT description from the following options related to nucleic acids: (A) A is mostly base paired with C (B) Structure of nucleotides are composed of six-member ring sugar, base and phosphate group. (C) The linkage between nucleotides is phosphodiester bond (D) Nucleic acids at neutral pH buffer (pH=7) are highly negatively charged (E) Quantification of DNA is based on absorbance 280nm