試題詳解

25. Gene targeting technologies have many applications, including
(A) inactivation of a gene locus by targeting gene disruption.
(B) replacing a wild type allele of a gene at one chromosome with a desirable mutant allele.
(C) to fuse a green fluorescent protein DNA sequence in frame after a gene of interest. The expression of resulting GFP fusion protein could be used to study how the gene of interest is regulated in vivo.
(D) to express Cre recombinase in a tissue specific manner by inserting Cre recombinase coding sequence downstream to a tissue specific enhancer sequence in genome
(E) none of above