11. Nucleosome core. Which of the following histone is not a core histone?
(A) H1
(B) H2A
(C) H2B
(D) H3
(E) H4

12. Antisense RNA and mRNA. Which of the following descriptions is not correct? (A) mRNA is made using the non-coding strand of DNA as a template. (B) mRNA is sense RNA. (C) IfRNA is made using the coding strand as a template, it is known as antisense RNA. (D) The sense and antisense strands of RNA can base pair. (E) Antisense RNA will base pair with the mRNA and enhance the translation.

13. Structure of tRNA.Which of the following descriptions is not correct? (A) A typical tRNA has 4 base-paired stems and 3 loops. (B) One stem is the 3' and 5' acceptor stem. . (C) One loop is the T- (or TφC) loop. (D) One loop is the D-1oop. (E) One loop is the codon loop.

14. The functions of translation initiation factor IF3 in bacteria. Which of the following descriptions is not correct? (A) IF3 binds to free 50S subunits. (B) IF3 prevents the 30S subunit from associating with 50S subunit. As an anti- association factor that causes a 30S subunit to remain in the pool of free subunits. (C) IF3 controls the ability of 30S subunits to bind to mRNA.30S must have IF3 to form initiation complexes with mRNA. (D) IF3 must be released from the 30S-mRNA complex in order for the 50S to join. (E) IF3 checks the accuracy of recognition of the first aminoacyl-tRNA and helps to direct it to the P site of the 30S subunit.

15. All chromatin remodeling complexes require a subunit for_________to  reorganize the nucleosomes. (A) histone binding (B) ATPase (C) repressor binding (D) DNAbinding (E) transcription factor binding

16. When working identifying factor involved in rRNA transcription, which of the following would you expect to bind to a chromatography column made of a resin conjugated to UBF1? (A) TFIB (B) TFIIE (C) TFIH (D) Sp1 (E) SL1

17. What component is not included in an operon? (A) operator (B) promoter (C) intron (D) both operator and promoter (E) both promoter and intron

18. What effects could extra acetyltransferase cause to an in vitro transcription assay? (A) There cause a complete repression of transcription. (B) It will cause a tighter association of histone with DNA, resulting in reduced transcription. (C) It will have no effect on the interaction of DNA with histones. (D) It will loosen the interaction of histone with DNA. (E) None of the choices are correct.

19. Abnormally oligomerized yeast protein Sup35 would lead to: (A) failure of translation to terminate. (B) abnormal chromosome inactivation by heterochromatin formation. (C) abnormal imprinting of some genes. (D) abnormal cytoskeletal structures. (E) failure of chromosomes to condense during mitosis.

20. Histone variants are closely related to one of the core histones. They can assemble into nucleosomes, the roles of histone variants are: (A) that are present during double-strand break repair. (B) that are disrupted during active transcription. (C) in centromeric regions. (D) that compact chromatin in sperm cells. (E) All of these are correct.

21. The effect a remodeling complex in transcription activation is: (A) histone deacetylation. (B) recruiting an repressor to a promoter. (C) DNA methylation. (D) displacing nucleosomes from a promoter. (E) breaking apart nucleosomes.

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