12. Assume that a lagging strand is made from the Region X, what will be the sequence of this lagging strand?
(A) 5'-CCAGTTAAC-3'
(B) 5'-GTTAACTGG-3'
(C) 5'-GGTCAATTG-3'
(D) 5'-CAATTGACC-3'

1. Which of the following explains the events in late stage of phage T7 infection in E. coli? (A) There is a high level of transcription of host genes. (B) Host polymerase holoenzyme directs transcription. (C) The host core enzyme participates in transcription in conjunction with the phage-encoded specific sigma factor. (D) Phage T7-specific core polymerase participates in transcription in conjunction with the host sigma factor. (E) The T7 phage -specific RNA polymerase specifies transcription of genes.

複選題2. Which of the following descriptions about infection of E. coli by phage λ is correct? (A) CII protects CIII against cellular proteases and thus is important for lytic cycle induction during a infection. (B) Q is an antiterminator that permits RNA polymerase to ignore the terminators at the ends of the immediate early genes and continue transcribing into the delayed early genes. (C) The cro gene is stimulated during the lytic cycle of infection. (D) N is an antiterminator which permits transcription of the late genes during the lytic cycle of infection (E) Without Q, transcription from the late promoter PR stops after 194 bp.

3. A DNA element that binds protein factors and stimulates transcription is called _____(A) Enhancer (B) Promoter (C) Suppressor (D) Operator (E) Terminator

4. Which of the following statement is correct regarding the sigma-factor? (A) Subregions 2.4 and 4.2 are involved in operator recognition. (B) Region 1 appears to be in preventing σ from binding to DNA by itself. (C) It determines rifampicin sensitivity or resistance. (D) Subregion 2.4 has a β-sheet domain. (E) Region 3 is the most highly conserved σ region.

5. During an experiment to study the rate of infection of bacteria with a phage, the bacterial cultures were accidentally exposed to a UV light source. Which of the following would be expected for this exposure? (A) recA gene is turned off. (B) Coprotease activity in repressor is activated. (C) Lysogenic cycle is induced. (D) Coprotease activity in RecA protein is activated. (E) SOS response is turned off.

6. Which of the following statements is not true concerning DNA replication in prokaryotes? (A) Semidiscontinuous (B) Semiconservative (C) RNA dependent. (D) There is one terminus of replication. (E) There is one origin of replication.

7. Which of the following is not a characteristic of the prokaryotic single stranded DNA binding (SSB) protein? (A) It binds more tightly to ssDNA than to dsDNA. (B) It aids helicase action. (C) It stimulates homologous DNA polymerase. (D) It keeps DNA strands from annealing. (E) It is not essential for cell replication.

8. Put the following steps of E. coli primosome assembly in the correct order. (1) DnaG binds. (2) DnaA binds to oriC at dnaA boxes. (3) DnaB binds to the open complex. (4) DnaA, ATP, and HU protein melt the DNA. (A) 2, 4, 1, 3 (B) 2, 4, 3, 1 (C) 4, 1, 2, 3 (D) 4, 2, 1, 3 (E) 3, 4, 1, 2

9. The Ds transposable element of maize cannot transpose on its own because it lacks (A) Tn3. (B) inverted repeats. (C) origin of replication. (D) direct repeats. (E) transposase.

10. Which of the following is not true concerning immunoglobulin gene recombination signal sequences? (A) There are conserved heptamers. (B) There are conserved nonamers. (C) Recombination only occurs between two heptamers. (D) The conserved sequences are separated by a nonconserved sequence of either a 12 bp or a 23 bp sequence. (E) Recombination occurs between a 12 bp signal and a 23 bp signal.

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