14. Which of the following is associated with silent genes in eukaryotic cells?
(A) Euchromatin
(B) Heterochromatin
(C) DNA hypersensitivity sites
(D) Histone tail acetylation
(E) Nucleosome-free zones

15. Eukaryotic mRNA splicing requires the presence of splicing signals in the intron. Which of the following is     not true     about the splicing signals? (A) The first two are GU (at the 5'-splice site) (B) The last two are AG (at the 3'-splice site) (C) A branch-point sequence is near the 3'end of an intron (D) A lariat branched intermediate forms by connecting GU and AG (E) A lariat branched intermediate forms by connecting G at 5'-splice site and A in the branch-point sequence

16. Which of the following snRNPs requires ATP for binding to its pre-mRNA substrate? (A) U1 (B) U2 (C) U4 (D) U5 (E) U11

17. Alternative splicing is a very common phenomenon in higher eukaryotes. Which of the following is     not     essential for the control of alternative splicing? (A) Exonic splicing enhancers (ESEs) (B) Exonic splicing silencers (ESSs) (C) Hu proteins (D) hnRNP AI (E) SR proteins

18. Which of the following is     true     for adding a tail in eukaryotic mRNA? (A) Increase half-life of mRNA (B) Increase turn-over rate of mRNA (C) Enhance transcription rate of mRNA (D) Inhibit mRNA splicing (E) Decrease half-life of mRNA

19. Which of the following is     not     a function of 5' capping of eukaryotic mRNA? (A) Protect mRNA from degradation (B) Enhance translatability of mRNA (C) Prevent transport of mRNA out of nucleus (D) Increase mRNA splicing (E) Decrease turn-over rate of mRNA

20. Which of the following is     not     involved in the generation of small interference (si) RNA? (A) Trigger ssRNA (B) Dicer (C) Ago2 (D) Release of guide strand (E) dsRNA

21. Which of the following is    not    involved in the generation of microRNA? (A) Stem-loop dsRNA precursor (B) RNA polymerase I (C) Drosha (D) Dicer (E) Ago2

22. Which of the following is    not    a part of post-transcriptional events in eukaryotes? (A) Histone methylation (B) RNA editing (C) Trans-splicing (D) 3' untranslated region (UTR)-dependent mRNA stability (E) 5' UTR-dependent mRNA stability

23. Which of the following is not a technique that can be used to study protein-protein interactions? (A) Protein microarrays (B) 2-D gel electrophoresis (C) Immunoaffinity chromatography (D) Phage display (E) Yeast two-hybrid analysis

24. DNA microarrays can be used to study (A) The DNA sequence of multiple chromosomes (B) The DNA sequence of multiple genomes (C) The clustering of expression of genes in time and space (D) Chromosomal rearrangements (E) Chromosomal abnormalities

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