試題詳解

17.The Agel restriction enzyme recognizes the sequence ACCGGT and cuts each strand between the first ( A ) and the second ( C )nucleotide. This can be represented as A|CCGGT, with the vertical bar indicating the cleavage position. If a DNA ,fragment is cut with Agel at both ends, cutting a plasmid with which of the following enzymes would then allow insertion ofthe fragment into the plasmid by simple ligation of sticky ends?
(A) MroI, with the recognition sequence T|CCGGA
(B) Aatl, with the recognition sequence AGG|CCT
(C) BstZI, with the recognition sequence C|GGCCG
(D) FseI, with the recognition sequence GGCCGG|CC
(E) None of the above