2. Which of the following enzymes will produce a blunt end (the cut site is indicated by the * in the recognition sequence)?
(A) Taql (T*CGA)
(B) Eagl (C*GGCCG)
(C) EcoRV (GAT*ATC)
(D) Nsil (ATGCA*T)

78. 家用冷氣機使用 R-32，而不用 R-134a 的主要理由，是因為 R-32 冷媒 (A)運轉壓力高 (B)飽和壓力低(C)蒸發潛熱大 (D)壓縮比低。

79. 更換壓縮機時，下列何者不需要考慮？ (A)庫內風扇是否正常 (B)R.S.C 是否正確 (C)焊接時需斷熱 (D)過載保護器是否匹配。

80. 使用鑽孔機時，不應使用下列何護具？ (A)棉紗手套 (B)耳塞 (C)防塵口罩 (D)護目鏡。

1. Telomerase (A) creates a short DNA sequence complementary to the DNA template. (B) creates a short RNA sequence complementary to the DNA template. (C) creates a short DNA sequence complementary to the RNA template. (D) solves the end replication problem in the leading strand.

3. DNA primase (A) creates a short RNA primer complementary to the RNA template. (B) creates a short DNA primer complementary to the RNA template. (C) creates a short DNA primer complementary to the DNA template. (D) creates a short DNA primer complementary to the DNA template.

4. The lagging strand is replicated with stretches of Okazaki fragments and that is why its synthesis is considered to be (A) discontinuous. (B) primed. (C) never stopping. (D) semiconservative.

5. A scientist developed a particular chemical to bind with adenine. If this chemical were applied prior to the S phase in the cell cycle, what might be a logical prediction of the outcome? (A) The DNA replication would proceed except that adenine would have the chemical bound to it and is incorporated into the new sister chromatids. (B) The DNA replication would proceed except that everywhere an adenine was supposed to be, a thymine was substituted into the new developing strand. (C) The DNA replication would proceed because cytosine and guanine would continue to form base pairs. (D) The DNA replication would stop because if adenine was bound then thymine would not have a complement with which to base pair.

6. As the two strands of DNA are unraveled, an enzyme relieves the strain on the two strands. The enzyme is (A) DNA polymerase III. (B) DNA polymerase I. (C) DNA helicase. (D) DNA gyrase. (E) DNA ligase.

7. Which of the following enzymes removes the primers used for DNA replication? (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III (D) DNA ligase

8. If you were able to look very closely at a portion of DNA and find methylated histones, you would (A) be mistaken since only DNA can be methylated, not histones. (B) be looking at a region of active chromatin. (C) be looking at a region of inactive chromatin. (D) be looking at transcription initiation.