複選題

2. Which physical or chemical factor of the following list can cause DNA mutation?
(A) Heat
(B) Ultra-violet light
(C) Ethidium bromide
(D) Nitrous acid
(E) 5-Bromouracil

6. Which physical or chemical factors in the following list can cause DNA mutation? (A) Heat (B) Ultra-violet light (C) Acridine (D) Nitrous acid (E) 5-Bromouracil

複選題1. Which physical or chemical factor of the following list can cause DNA mutation? (A) Heat (B) Ultra-violet light (C) Ethidium bromide (D) Nitrous acid (E) 5-Bromouracil

複選題2. Which physical or chemical factor can cause DNA mutation? (A) Heat (B) Cold (C) Ultra-violet light (D) Ethidium bromide (E) Nitrous acid

複選題3. U-rich small nuclear RNAs (snRNAs) are important for base pairing with the pre-mRNA and are required for pre-mRNA splicing. They include (A) U1 (B) U2 (C) U3 (D) U4/U6 (E) U5

複選題4. Which organism listed below would need spliceosome-based pre-mRNA splicing to remove introns? (A) Homo sapiens (B) Drosophila melanogaster (C) Caenorhabditis elegans (D) Saccharomyces cerevisiae (E) Escherichia coli

複選題5. MicroRNAs are small non-coding regulatory RNAs that down regulate gene expression by the mechanisms including (A) Repress translation of target mRNAs (B) Trigger cleavage of target mRNAs (C) Trigger decapping of target mRNAs (D) Enhance polyadenylation of target mRNAs (E) Accumulate targets mRNAs in the P-bodies for storage or degradation

6. RNA interference (RNAi) is an evolutionarily conserved mechanism to degrade RNA molecules in a sequence-specific manner. This mechanism does NOT exist in (A) Homo sapiens (B) Drosophila melanogaster (C) Caenorhabditis elegans (D) Saccharomyces cerevisiae (E) Escherichia coli

7. During the first stage of translation, the small and large ribosomal subunits assemble around an mRNA that has an aminoacylated initiator tRNA correctly positioned at the start codon. The process is mediated by a special set of proteins known as translation initiation (IFs). In eukaryotes, the translation initiation complex contains the 40S subunit, the Met-tRNA and IFs      NOT     including (A) eIF1 (B) eIF2 (C) eIF3 (D) eIF4 (E) eIF6

複選題8. Which of the following techniques can be used to measure the amount of a specific mRNA in a sample? (A) RT-real time quantitative PCR (B) Northern blot analysis (C) Southern blot analysis (D) Western blot analysis (E) RNase protection assay

9. Scott wants to set up a RT-real time quantitative PCR experiment. Which enzyme(s) will he need? (A) E. coli DNA polymerase I (B) T4 DNA ligase (C) Taq DNA polymerase (D) Reverse transcriptase (E) T7 RNA polymerase

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