21. Based on the mammalian phylogeny shown in Figure 1, which of the following statements are true? (select all that apply)
(A) Marsupialia is much more ancient than all other mammalian lineages.
(B) Marsupialia is phylogenetically more closely related to Afrotheria than to Rodentia.
(C) Chiroptera is phylogenetically more closely related to Perissodactyla than to Primates.
(D) Lagomorpha is the sister group to Rodentia.
(E) The rate of nucleotide evolution in Primates is much faster than in Lagomorpha.

1. Which of the following is NOT considered a valid line of scientific evidence supporting biological evolution? (A) The ability of individuals to adapt to their environment during their lifetime. (B) The fossil record showing transitional forms and changes in species over geological time. (C) Homologous structures in different species, indicating common ancestry. (D) Similarities in early embryonic development across vertebrate species. (E) Molecular similarities, such as shared DNA sequences and proteins among organisms.

2. The Hardy-Weinberg principle states that allele and genotype frequencies in a population will remain constant from generation to generation if certain conditions are met. Which of the following is one of the required assumptions for a population to be in Hardy-Weinberg equilibrium? (A) Natural selection favors heterozygotes. (B) Mutations occur at equal rates in both directions. (C) The population is infinitely large and mating is random. (D) Allele frequencies change slowly over time. (E) Individuals acquire beneficial traits during their lifetime.

3. In a population that is in Hardy-Weinberg equilibrium. For a locus with two alleles, A and a. If the allele frequency of A is 0.4. What is the frequency of Aa heterozygotes? (A) 0.04 (B) 0.16 (C) 0.24 (D) 0.48 (E) 0.36

4. Which of the following descriptions is incorrect for a recognized type of natural selection? (A) Directional selection: A mode of natural selection in which individuals with phenotypes at one extreme of a trait distribution have higher fitness than those with intermediate or opposite extreme phenotypes. (B) Stabilizing selection: A mode of natural selection that favors both phenotypic extremes over intermediates, potentially increasing variation and leading to a bimodal distribution or speciation. (C) Balancing selection: A mode of natural selection that maintains genetic diversity in a population by favoring multiple alleles, often through mechanisms like heterozygote advantage or varying selection pressures across environments. (D) Purifying selection: A mode of selection that removes deleterious mutations or alleles, preserving functional genetic sequences and reducing genetic variation at conserved loci. (E) Frequency-dependent selection: A mode of selection where a phenotype's fitness depends on its frequency in the population—often, rare phenotypes have higher fitness.

5. Which of the following situations is best explained by the Biological Species Concept? (A) Two populations of frogs live in the same habitat and overlap in breeding season, but mating between them produces no viable or fertile offspring due to intrinsic reproductive barriers. (B) Fossil shells from different sedimentary layers show gradual changes in shape over millions of years, allowing paleontologists to distinguish "species" based on morphology. (C) Two groups of bacteria are classified as different species because they have less than 70% DNA similarity and differ in several metabolic pathways. (D) Closely related orchid varieties are considered separate species because each is pollinated exclusively by a different insect species, even though they could produce fertile hybrids in a greenhouse. (E) Two populations of birds have diverged genetically on different islands and form a monophyletic group on a phylogenetic tree, distinct from all other birds.

6. Continuing from Question 5, which of the situations above is best explained by the Phylogenetic Species Concept? (A) A (B) B (C) C (D) D (E) E

7. Which of the following is an example of gametic isolation? (A) Two fern species with overlapping ranges produce hybrid spores that germinate poorly and fail to develop into viable gametophytes due to differences in ploidy. (B) In sympatric frog populations, one species calls only at night while the other calls during the day, preventing mating attempts. (C) Pollen grains from one plant species land on the stigma of another species but fail to germinate. (D) Two populations of lizards on adjacent islands rarely meet due to geographic separation by ocean barriers. (E) Two bird species have distinct courtship dances; females mate only with males performing the correct display.

8. Continuing from Question 7, which of above scenarios is an example of postzygotic isolation? (A) A (B) B (C) C (D) D (E) E

9. Which statement best supports the endosymbiotic theory for the origin of mitochondria and chloroplasts? (A) They are found only in multicellular eukaryotes. (B) They contain their own linear DNA similar to nuclear chromosomes. (C) They divide asexually by mitosis within eukaryotic cell. (D) They have circular DNA and 70S ribosomes. (E) Mitochondria and chloroplasts are surrounded by a single membrane, similar to the plasma membrane of some prokaryotes.

10. Which of the following eukaryotic groups lacks a multicellular stage with cellular differentiation in their life cycle? (A) Animals (Metazoa) (B) Land plants (Embryophyta) (C) Red algae (Rhodophyta) (D) Brown algae (Phaeophyta) (E) Diatoms (Diatomeae)

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