試題詳解

25. Adenosine deaminase (ADA) deficiency is an inherited disorder that damages the immune system and causes severe combined immunodeficiency (SCID). All of the following steps were performed for gene therapy when a patient with defective ADA was treated. Except?
(A) Human leukocyte antigen (HLA) testing should be performed before gene therapy.
(B) Haematopoietic stem cells were collected from the patient's bone marrow.
(C) CD34 positive cells were transduced with a viral vector expressing functional ADA.
(D) The transfected cells were grown in culture to ensure ADA gene is active.
(E) The transfected cells are reinfused into the same patient.