27. High throughput sequencing technologies become powerful tools to study molecular biology on a genome-wide scale. Which of the following sequencing techniques is used to assess the translational status?
(A) RNA binding protein immunoprecipitation-sequencing (RIP-Seq).
(B) Chromatin immunoprecipitation-sequencing (ChIP-Seq).
(C) Global run-on sequencing (GRO-Seq).
(D) 4-thiouridine sequencing (4sU-Seg).
(E) Ribosome sequencing (Ribo-Seq).

23 Which of the following statements is correct regarding translation termination in eukaryotes? (A) The structure and amino acid sequence of the Class I eukaryotic release factors (eRFs) are similar with those of the Class I release factors in prokaryotes. (B) eRF1 induces release of the nascent polypeptide from the peptidy1-transferase center. (C) Rlil is an ATP-binding protein and brings eRF 1 to the ribosome. (D) eRF3 is a GTP-binding protein and catalyzes release of eRF1 from the A-site. (E) Neither eRF1 nor eRF3 participates in separation of the large and small ribosomal subunits after release of the nascent polypeptide.

24. Both the catabolite activator protein (CAP) and the Lac repressor can bind DNA, but why does CAP facilitate transcription while the Lac repressor represses transcription? (A) CAP and the Lac repressor use different structural motifs to bind DNA. (B) CAP binds to the region overlapping the promoter, while the Lac repressor binds to the region at a distance from the promoter. (C) The Lac repressor can interact with the RNA polymerase to inhibit transcription. (D) CAP has an activating surface that recruits the RNA polymerase. (E) CAP binds as a homodimer, while the Lac repressor binds as a homotetramer.

25. Which of the following is the key bacteriophage X regulatory protein that acts as an activator and establishes lysogeny upon infection of a new host? (A) CI. (B) CI. (C) CIII. (D) Cro. (E) Q

26. Which of the following DNA binding domains or motifs is found in most bacterial regulatory proteins? (A) Zinc-finger domain. (B) Basic leucine zipper motif. (C) AT-hook motif. (D) Helix-turn-helix motif. (E) Helix-loop-helix motif.

複選題28. Which of the following statements is correct regarding riboswitches? (A) Most riboswitches are trans-regulatory elements. (B) Riboswitches can regulate gene expression at both transcriptional and translational levels. (C) The riboswitch region that binds the small-molecule ligand is called the receptor. (D) Riboswitches are typically found downstream of genes involved in the synthesis of the metabolite ligand recognized by the riboswitch. (E) Riboswitches can mediate rho-dependent transcription termination.

複選題29. Which of the following statements is correct regarding clustered regularly interspaced short palindromic repeats (CRISPR)? (A) CRISPR is found in the eukaryotic genome. (B) A CRISPR locus consists of 2 major components: repeated and spacer sequences. (C) The spacer sequences are acquired from infecting viruses. (D) Cas1 and Cas2 are involved in processing of CRISPR RNA (crRNA). (E) The CRISPR/Cas6 system is currently the most widely utilized tool for genome

30. Which of the following statements is correct regarding small RNA-mediated silencing pathways in eukaryotes? (A) Piwi-interaction RNAs (piRNAs) are expressed predominantly in vegetative cells. (B) Both small interfering RNAs (siRNAs) and microRNAs (miRNAs) are generated from longer RNA molecules by Dicer. (C) The major component of the RNA-induced silencing complex is called Drosha. (D) The size of small RNAs is typically in the range of 30-40 nucleotides. (E) The miRNA is typically highly specific with only one mRNA target. editing.

複選題31. Which of the following statements are correct regarding the central dogma of molecular biology, as firstly stated by Francis Crick in 1957? (A) The sequential information can be transferred from DNA to RNA. (B) The sequential information can be transferred from RNA to DNA. (C) The sequential information can be transferred from RNA to protein. (D) The sequential information can be transferred from protein to RNA. (E) The sequential information can be transferred from protein to protein.

複選題32. Which of the following histones form a tetramer in the first step of the assembly of a nucleosome? (A) H1. (B) H2A. (C) H2B. (D) H3. (E) H4.

複選題33. Which of the following features are correct regarding the mechanism of DNA polymerase? (A) DNA polymerase uses a single active site to catalyze the addition of any of the four deoxynucleoside triphosphates. (B) Steric constraints prevent DNA polymerase from using ribonucleoside triphosphates as substrate. (C) The thumb domain of DNA polymerase is intimately involved in catalysis. (D) Processivity is a crucial character for DNA polymerase and defines the average number of nucleotides added per template binding event. (E) Removal of incorrectly base-paired nucleotides is mediated by intrinsic endonuclease activity of DNA polymerase.