3. You are designing an experiment to investigate the presence of promoter binding sites for holoenzymes bearing in B. subtilis. A DNA template is available along with the two holoenzyme complexes and the in vitro transcription buffers. Which of the following is a suitable negative control for this experiment? (A) a reaction containing all the components minus UTP (B) a reaction containing DNA lacking the -10 region (C) a reaction containing DNA lacking the -35 region (D) a reaction containing DNA lacking the UP element (E) a reaction containing a DNA lacking the two promoter regions