試題詳解

30. Imagine you want to study a human crystallin protein. To obtain a sufficient amount of this protein, you decide to clone its gene. Assuming you know the gene sequence, which approach would allow you to produce the protein in a suitable system like bacteria?
(A) Isolate lens cells, extract genomic DNA, and insert the entire gene directly into a bacterial expression vector.
(B) Isolate lens cells, extract mRNA, use reverse transcriptase to make cDNA, and insert the cDNA into a bacterial expression vector.
(C) Directly synthesize the protein from its known amino acid sequence using a chemical synthesizer.
(D) Use PCR to amplify the gene from a brain cell and insert it into a eukaryotic yeast expression vector.