35. Which of the following statements is incorrect?
(A) 2, 3, and 5 are incorrect.
(B) 1, 3, and 4 are incorrect.
(C) 1,3, and 5 are incorrect.
(D) All are incorrect.
(E) None is incorrect.

31. Which of the following is not correct concerning cooperative binding of a ligand to a protein? (A) It is usually a form of allosteric interaction. (B) It is usually associated with proteins with multiple subunits. (C) It results in a sigmoidal binding curve. (D) It rarely occurs in enzymes. (E) It results in a nonlinear Hill Plot.

32. In the following diagram of the first step in the reaction catalyzed by the protease chymotrypsin, the process of general base catalysis is illustrated by the number__________ , and the process of covalent catalysis is illustrated by the number ____________.(A) 3；2 (B) 1；3 (C) 2；3 (D) 2； 1

33. The steady state assumption, as applied to enzyme kinetics, implies: (A) the ES complex is formed and broken down at equivalent rates. (B) the enzyme is regulated. (C) Km=Ks. (D) the Km is equivalent to the cellular substrate concentration. (E) the maximum velocity occurs when the enzyme is saturated. 

34. Which one of the following statements is correct? 1. Conversion of ethanol to acetaldehyde is a two-electron oxidation.  2. FAD is a stronger reducing reagent than NAD+.  3. There is entropy loss when ADP is phosphorylated.  4. Creatine phosphate is a high-energy-bond containing molecule.  5. NAD+ is the electron acceptor during in the conversion of 3-phosphoglycerate to 1,3-bisphosphoglycerate. (A) 1, 2, and 3 are correct. (B)2, 3, and 4 are correct. (C)1, 3, and 4 are correct. (D) 1,3, 4, and 5 are correct. (E)2, 3, 4, and 5 are correct.

36. Which statement regarding glycogen breakdown is correct?  1. Cleavage of glycogen terminal glucose is catalyzed by glycogen phosphorylase.  2. The breaksdown of glycogen to generate Glucose-6-phosphate needs no ATP. 3. Transferase activity in glycogen debranching enzyme catalyzes the transfer of shorter α(1→)6 linked glucose chain to another o(1-)6 linked glucose chain for further cleavage reaction. 4. Glucosidase activity in glycogen debranching enzyme catalyzes the cleavage ofα(1→6) linked glucose for complete breaking down the branch in glycogen (A) 1, 2, and 3 are correct. (B) 2, 3, and 4 are correct. (C) 1, 2, and 4 are correct. (D) All are correct. (E) None is correct.

37. Which one of the following statements is incorrect? (A) P-type ATPase transporter is inhibited by vanadate. (B) Cyanide (CN-) blocks the lactose transporter in E.coli. (C) Tetradotoxin impairs voltage-gated Na+ channels. (D) Statin competes with acetyl Co-A. (E) Aspirin inhibits COX-2. pyruvatc

38. Which one is incorrect? (A) Catabolisin of fatty acids produces acetyl-CoA. (B) Catabolism of fatty acids produces reducing power NADH. (C) Anabolism of fatty acids requires acetyl-CoA and malonyl-CoA. (D) Anabolism of fatty acids requires reducing power from NADPH. (E) Fatty acid synthase contains a domain of acyl-CoA dehydrogenase.

39. Which one is not a rate-limiting step in lipid metabolism? (A) Carnitine acytransferase I for fatty acid entry into mitochondria (B) Acetyl-CoA carboxylase for biosynthesis of fatty acid (C) PEP carboxykinasc for triacylgiycerol cycle (D) Acyl transferase for phospholipid synthesis (E) HMG reductase for cholesterol synthesis

40. Which of the following descriptions is(are) false?  1. The difference between a ribonucieotide and a deoxyribonucleotide is that a ribonucleotide has an --OH instead of an -H at C-2.  2. The DNA copies of mRNA found in a cDNA libtary are made by reverse transcriptase.  3. Type II restriction enzymes cleave DNA only at recognition sequences specific to a given restriction enzyme 4. Deamination of the nucleotide base 5-methylcytosine yields nucleotide base thymine. 5. Type II restriction enzymes may make staggered (off-center) cuts within their recognition scquences. (A) 5 only (B) 3 only (C) 4 only (D) 1 and 2 (E) Nonc of the descriptions are false.

41. Which of the following descriptions is false? (A) If clectron transfer in tightly coupled mitochondria is blocked (with antimycin A) between cytochrome b and cytochrome cl, O2 uptake will continue. (B) A drug that inhibits the ATP synthase in the mitochondrion will also inhibit the flow of electrons down the chain of carriers. (C) Oxidative phosphorylation can not occur in the absence of an intact inner mitochondrial membrance. (D) Uncoupling of mitochondrial oxidative phosphorylation haits mitochondrial ATP formation, but allows continued O2 consumption. (E) If electron transfer in tightly coupled mitochondria is blocked (with antimycin A) between cytochrome b and cytochrome cl, then all ATP synthesis will stop.