40. The first step in the secretory pathway that should be inhibited by a dominant-negative mutant of SNARE proteins is
(A) rouigh endoplasmic reticulum to Golgi transport.
(B) intra Golgi transport.
(C) trans Golgi network (TGN) transport to the plasma membrane.
(D) TGN transport to the late endosome.

36. Steroid hormones activate transcription by (A) binding to a nuclear receptor. (B) phosphorylating a protein kinase. (C) binding to specific cell-surface receptors. (D) inhibiting a histone deacetylase.

37. Transcriptionally inactive genes (A) are not located within nucleosomes. (B) often are methylated. (C) are not resistant to DNase I. (D) are always located within euchromatin.

38. What is the function of TFIIH in the transcription initiation complex? (A) binding to the TATA box (B) unwinding the DNA duplex (C) catalyzing the synthesis of RNA (D) all of the above

39. Protein X contains 7 transmembrane domains, with a short N-terminus and a long C-terminus. Following protein synthesis, the N-terminus of the protein faces the lumen (the inside) of endoplasmic reticulum (ER). After protein X is transported to the cell surface via exocytosis, you would expect the C-terminus of the protein X to be (A) extracellular. (B) cytoplasmic. (C) in the lumen ofER. (D) in the lipid bilayer of the plasma membrane.

1. RNA is involved in many cellular processes. Which method is not used for RNA detection? (A) Real-time PCR (B) Fluorescence in situ hybridization (C) Northern blotting (D) Footprinting (E) cDNA microarray

2. Which is incorrect about eukaryotic mRNA? (A) Transcribed by RNA polymerase II (B) Contain 5' cap and 3' poly( A ) tail (C) Contain 5'UTR to regulate translation efficiency (D) Contain 3'UTR to be targeted by microRNAs (E) Cannot be modified

3. Which is incorrect about genomic imprinting? (A) Imprinting genes show mono-allelic, parent-specific gene expression. (B) All imprinting genes encode proteins. (C) It is epigenetically regulated in imprinting control regions (ICR). (D) ICR is under DNA methylation in the CS position of a cytosine residue, forming 5-methylcytosine (5 mC). (E) Prader-Willi syndrome is a known imprinting disorder.

4.Which part of the ribosome is responsible for decoding? (A) LSU (B) SSU (C)mRNA (D) tRNA (E)SiRNA

5. The initiation phase of the translation involves binding which component to mRNA? (A) LSU (B) SSU (C)IncRNA (D) mitochondria (E) RNase A

6. Which organelle is responsible for protein degradation? (A) Mitochondria (B) Ribosome (C) Lysosome (D) Centriole (E) Endoplasmic reticulum