61. Which of the followings are used to determine the primary structure of proteins?
① size exclusion chromatography ② SDS polyacrylamide gel electrophoresis ③ Edman degradation ④ circular dichroism ⑤ analysis of amino acid composition
(A) ①②
(B) ②③
(C) ③④
(D) ④⑤
(E) ③⑤
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