69. How many of the followings are ketogenic amino acid in human? Asp； Phe； Arg； Val； His； Thr； Lys.
(A) 2
(B) 3
(C) 4
(D) 5
(E) 6

複選題65. X uses a Y to link cofactor, Z. Which one of the followings is NOT true? (A) X: Pyruvate dehydrogenase； Y: lysine residue； Z: lipoamide (B) X: Aconitase； Y: cysteine residue； Z: iron-sulfur center (C) X: Pyruvate carboxylase； Y: lysine residue； Z: biotin (D) X: Acyl carrier protein； Y: cysteine residue； Z: pantothenate (E) X: Aminotransferase； Y: lysine residue； Z: pyridoxal phosphate

66. Which of following statements is NOT true regarding the lysozyme’s Phillips mechanism? (A) Glu 35 acts as both “general acid” and “general base” catalysis. (B) The active site of lysozyme can take six residues of monosaccharides. (C) The D ring of monosaccharide is distorted into boat form conformation. (D) The oxonium ion transition state is stabilized by Asp 52. (E) The optimal pH of this reaction is between 4 and 6.

67. Enzyme X follows Michaelis-Menten kinetics. As shown in the figure, its value of Vmax and Km can also be obtained by using Eadie-Hofstee plot. The initial reaction rate is v0 with the substrate concentration [S]. What is the intercept on v0/[S] axis?(A) Vmax/Km (B) 1/Km (C) Km (D) Vmax (E) 1/Vmax

68. Among 9 different molecules below, X of them can cleave disulfide bond, and Y of them can react with the amino group of L-amino acids. Therefore, X + Y =__________ .cyanogen bromide； iodoacetate； phenylisothiocyanate； β-mercaptoethanol； performic acid； 9 -fluorenylmethoxycarbonyl chloride； dithiothreitol；； guanidinium hydrochloride； urea. (A) 2 (B) 3 (C) 4 (D) 5 (E) 6

70. How many items of the followings have the activity of proof-reading to reduce the error rate in biosynthesis?DNA polymerase； RNA polymerase II； amino-acyl tRNA synthetase； Initiation Factor-1； polyketide synthase； Elongation Factor-Tu； Klenow fragment. (A) 2 (B) 3 (C) 4 (D) 5 (E) 6

【已刪除】71. Which one of followings is NOT used for thymine dimer repair? (A) Daughter strand gap repair (B) Base excision repair (C) Nucleotide excision repair (D) Photolyase (E) DNA polymerase Eta (Pol )

72. The pentose phosphate pathway of glucose oxidation produces ______. (A) coenzyme Q10 (B) FADH2 (C) glucose-1-phosphate (D) NADH (E) ribose-5-phosphate

73. The thickness of endoplasmic reticulum membrane is about 37.5 Å. Which one of the followings is closest to this length? (A) alpha-helix with 10 amino acid residues long (B) alpha-helix with 40 amino acid residues long (C) alpha-helix with 80 amino acid residues long (D) B-DNA with 10 base pairs long (E) B-DNA with 40 base pairs long

74. There are cholesterols and cholesterol esters in LDL. How and where are cholesterol esters generated in a human body? (A) Cholesterol esters are generated by lecithin: cholesterol acyltransferase in endothelial cells. (B) Cholesterol esters are produced by lecithin: cholesterol acyltransferase in macrophages. (C) Cholesterol esters come from the reaction between free short-chain fatty acids and cholesterols. (D) Cholesterol esters are generated from the reaction between free long-chain fatty acids and cholesterols. (E) Cholesterol esters are produced by lecithin: cholesterol acyltransferase in the bloodstream.

75. Okazaki fragments are ______. (A) short DNA pieces that explain how DNA is synthesized on the lagging strand (B) short DNA pieces that explain how DNA is synthesized on the leading strand (C) the remnants of the original strands that are dispersed in the new double stranded DNA molecules (D) RNA primers used for DNA replication (E) Fragments of mRNA that are processed into mature mRNA molecules