試題詳解

72. The molecular weight of Protein A (pI = 4.0), B (pI = 6.8), and C (pI = 8.5) is 14 kDa, 24 kDa, and 30 kDa, respectively. All of them are monomer proteins. Which of the following statements is CORRECT?

(A) Protein A elutes out first, when you use gel filtration column to separate the mixture of those three proteins.
(B) Protein C is the one that moves fastest, when you separate the mixture of those three proteins by SDS-PAGE.
(C) As shown in the right figure, you can use this isoelectric focusing apparatus to separate those three proteins.
(D) When you use a DEAE ion exchange column and 10 mM Tris-Cl, pH 7.0 buffer to separate these three proteins, Protein A will be bound by this column.
(E) None of the above