76. What two classes of enzymes play an important role in generating and propagating a recombinant DNA molecule in DNA cloning?
(A) recombinases and DNA polymerase α
(B) recombinases and DNA polymerase γ
(C) restriction endonucleases and DNA ligases
(D) restriction endonucleases and recombinases
(E) restriction endonucleases and reverse transcriptase

72. Which of the following statements regarding yeast two-hybrid analysis is false? (A) The goal is to bring together the DNA-binding domain and the activation domain of the yeast Gal4 protein. (B) Gal4 protein has two domains: one that binds a specific DNA sequence domain, the other activates RNA polymerase to synthesize tRNA from an adjacent gene (reporter gene). (C) A library can be set up to fuse to the Gal4 protein DNA-binding domain. (D) The interacting protein can be quickly identified by DNA sequencing of the fusion protein′s gene. (E) Some false positive results occur, due to the formation of multiprotein complexes.

73. What amino acid residues in some membrane-associated proteins are covalently linked to myristoyl or farnesyl groups? (A) asparagine and lysine (B) cysteine and glycine (C) serine and tyrosine (D) asparagine and serine (E) proline and lysine

74. What main component in plasma membrane has the oligosaccharide head groups to determine human blood types (O, A, B, and AB)? (A) caveolin in lipid raft (B) clathrin in apical surface (C) sphingolipid (D) phospholipid (E) cholesterol

75. What enzyme is frequently used for the measurement of the concentrations of glucose in blood? (A) glucokinase (B) glucose epimerase (C) glucose dehydrogenase (D) glucose dehydration (E) glucose oxidase

77. Insulin exerts its action through plasma membrane receptors to regulate the level of blood glucose. An elevated insulin level in the blood _______. (A) results from a below-normal blood glucose level (B) inhibits glycogen synthesis in liver and muscle (C) stimulates synthesis of fatty acids in liver and triacylglycerols in adipose tissue (D) inhibits glucose uptake by liver (E) stimulates glycogen breakdown in liver

78. Which of the following statements regarding polysaccharide is correct? (A) The glucose residues are linked by (β1→4) glycosidic bonds in cellulose. (B) In bacteria, dextrans are made up of (α1→4)-linked poly-D-glucose. (C) Chitin is a linear heteropolysaccharide composed of N-acetylglucosamine. (D) Glycogen is a polymer of glucoses with (α1→4)-linked branches. (E) Amylose is a branched glucose polymer.

79. Which of the following amino acids belong to ketogenic and glucogenic? (a) Trp (b) Phe (c) Tyr (d) Arg (e) Met (A) (a) (c) (e) (B) (a) (b) (c) (C) (b) (c) (e) (D) (b) (d) (e) (E) (a) (d) (e)

80. Which of the following statements regarding biosynthesis of fatty acids is correct? (A) NADH is required in the biosynthesis of fatty acids. (B) Fatty acids synthase I in vertebrates consists of 4 polypeptide chains. (C) Fatty acids synthase I is not a homodimer molecule. (D) HCO3 - is involved in biosynthesis of fatty acids. (E) There are 8 active sites in fatty acids synthase I.

81. Which of the following statements about Klenow fragment is false? (A) Klenow fragment is derived from E. coli DNA polymerase I. (B) It contains DNA polymerase activity. (C) It contains 3’→5’ exonuclease activity. (D) Klenow fragment is derived from large fragment of E. coli RNA polymerase I. (E) It is lack of 5’→3’ exonuclease activity.

82. Which of the following statements regarding β-oxidation of fatty acids is correct? (A) 16 acetyl-CoA molecules are produced by β-oxidation of palmitate. (B) One acetyl group is removed in one turn of β-oxidation. (C) In β-oxidation, ATP can be produced. (D) CO2 is generated by β-oxidation of palmitate. (E) The β-oxidation of unsaturated fatty acids has 4 basic steps.