試題詳解

8.Which of the following descriptions about electrophoretic mobility shift assay (EMSA) is not correct?
(A) It is used to detect protein complexes with nucleic acids.
(B) Solutions of protein and nucleic acid are combined and the resulting mixtures are subjected to electrophoresis under native conditions through polyacrylamide or agarose gel.
(C) The distribution of species containing nucleic acid is deternined usually by autoradiography of 32P-labeled nucleic acid
(D) Protein-nucleic acid complexes migrate more faster than the corresponding free nucleic acid.
(E) The electrophoretic mobility of a complex provides little direct information about the location of the nucleic acid sequences that are occupied by protein.