84. Two proteins have similar molecular masses (protein A: isoelectric point 5.8, 50 kDa; protein B: isoelectric point 8.5, 50.5 kDa). Which technique would most effectively separate them?
(A) Size-exclusion chromatography
(B) Ion exchange chromatography
(C) SDS-PAGE
(D) Ultracentrifugation
(E) X-ray crystallography

1. In the cell reproduction, which checkpoint ensures that DNA replication is complete before mitosis proceeds? (A) G₂ checkpoint (B) G₁ checkpoint (C) S checkpoint (D) Metaphase checkpoint (E) Cytokinesis checkpoint

2. An individual whose genitalia and internal ducts are male but whose testes are underdeveloped and do not produce sperms is most likely a victim of _____. (A) Down syndrome (B) Turner syndrome (C) Patau syndrome (D) Klinefelter syndrome (E) Edwards syndrome

3. In vascular plants, lateral meristems are primarily responsible for _____. (A) primary growth in length (B) secondary growth in thickness (C) leaf development (D) flower formation (E) seed germination

4. Bryophytes differ from vascular plants primarily because bryophytes _____. (A) produce seeds (B) possess lignified vascular tissue (C) require water for fertilization (D) have dominant sporophyte generations (E) produce pollen

5. Double fertilization in angiosperms results in the formation of _____. (A) one diploid embryo and one triploid endosperm (B) two diploid embryos (C) two triploid embryos (D) one haploid embryo (E) one diploid embryo only

6. In vertebrates relying on extracellular digestion, which intestinal feature most directly enhances the rate of nutrient uptake across the epithelial barrier? (A) Slowness of intestinal transit time (B) Maintenance of low luminal pH levels (C) Increased thickness of intestinal muscle layers (D) Emulsifying action of luminal bile salts (E) Expansion of epithelial surface area

7. Which characteristic is correct between the cell walls of bacteria and archaea? (A) Archaea cell walls are primarily made of polysaccharide, while bacteria use lipopolysaccharide. (B) Bacterial cell walls contain peptidoglycan, which is lacking in the cell walls of archaea. (C) Archaea lack a cell wall. (D) Both domains use peptidoglycan as the primary structural component of their walls. (E) Cell walls of archaea are structurally similar to eukaryotic cell walls.

8. Which of the following statements about immune responses is correct? (A) Phagocytic white blood cells can specifically recognize and destroy particular bacteria or viruses. (B) During an allergic reaction, monocytes release histamine, causing skin redness and swelling. (C) During inflammation, red blood cells leak out of blood vessels, causing redness, heat, swelling, and pain. (D) In the organ transplantation, T cells mediate a cellular immune response leading to rejection. (E) Passive immunity typically lasts for several years without diminishing.

9. High-level transcription in eukaryotes relies on the interaction between activators bound to distant enhancers and the transcription machinery at the promoter. Which statement best describes the structural change in DNA that facilitates this interaction? (A) The DNA acts as a track for activators to slide toward the promoter. (B) The DNA is permanently unwound to reduce distance. (C) The DNA loops to connect activators with the promoter. (D) The DNA is spliced to remove intervening sequences. (E) The DNA is chemically modified with RNA-like caps to signal initiation.

10. Which structures are found exclusively in birds? (A) Carina and syrinx (B) Cloaca and feathers (C) Epidermal scales and beak (D) Gizzard and crop (E) Wings and hollow bones

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