阿摩線上測驗
題組內容
4.25%
Fruit ripening is a complex and genetically programmed process modul ulated by transcription factors, hormones, and other regulators. However, the mechanism underlying the regulatory loop involving the membrane-protein targets of RIPENING-INHIBITOR (RIN) remains poorly understo ood. To unravel the function of tomato (Solanum bycopersicum) FERONIA Like (SIFERI), a putative MADS-box transcription factor target gene, we investigated and addressed the significance of SIFERL in fruit ripening by combining reverse genetics, biochemical, and cytological analyses. Here, we report that RIN and Tomato AGAMOUS LIKE1 (TAGLi) directly bind to the promoter region of SIFERL and further activate its expression transcriptionally, of SIFERI signifcantly accelerated the ripening process of tomato fruit, whereas RNA interrersicn suggesting a potential role ofSIFERLin fruit ripening. Overexpression knockdown of SIFERL resulted in delayed fruit ripening. Moreover, a surface plasmon resonance assay coupled with tandem mass spectromctry and a protein interaction assay rcvealed that SIFERL interacts with the key enzyme S-adenosyl-Met synthetase 1 (SISAMS1) in the ethylene biosynthesis pathway, leading to increased S-adenosyl-Met accumulation and elevated ethylene production. Thus, SIFERL serves as a positive regulator of cthylene production and fruit ripening. This study provides clues to the molecular regulatory networks underiying fruit ripening. (Plant Physiology, Published December 2020. DOI https://doi.org/10.1104/pp.20.01203)
Fruit ripening is a complex and genetically programmed process modul ulated by transcription factors, hormones, and other regulators. However, the mechanism underlying the regulatory loop involving the membrane-protein targets of RIPENING-INHIBITOR (RIN) remains poorly understo ood. To unravel the function of tomato (Solanum bycopersicum) FERONIA Like (SIFERI), a putative MADS-box transcription factor target gene, we investigated and addressed the significance of SIFERL in fruit ripening by combining reverse genetics, biochemical, and cytological analyses. Here, we report that RIN and Tomato AGAMOUS LIKE1 (TAGLi) directly bind to the promoter region of SIFERL and further activate its expression transcriptionally, of SIFERI signifcantly accelerated the ripening process of tomato fruit, whereas RNA interrersicn suggesting a potential role ofSIFERLin fruit ripening. Overexpression knockdown of SIFERL resulted in delayed fruit ripening. Moreover, a surface plasmon resonance assay coupled with tandem mass spectromctry and a protein interaction assay rcvealed that SIFERL interacts with the key enzyme S-adenosyl-Met synthetase 1 (SISAMS1) in the ethylene biosynthesis pathway, leading to increased S-adenosyl-Met accumulation and elevated ethylene production. Thus, SIFERL serves as a positive regulator of cthylene production and fruit ripening. This study provides clues to the molecular regulatory networks underiying fruit ripening. (Plant Physiology, Published December 2020. DOI https://doi.org/10.1104/pp.20.01203)