四、電梯困人時，應該依相關標準作業程序進行救援作業，請簡述無機房式電梯停止於樓層 中間困人之手動機械釋放煞車緊急救援流程。

二、問答題1. Draw a diagram of the scanning model of translation initiation. Present evidence that a scanning ribosome can bypass an AUG and initiate at a downstream AUG. 

2. How do we know that mRNAs are read in the 5'→ 3' direction? 

三、翻譯題The template strand of the DNA is copied during transcription, and its sequence is the complement of the RNA transcript. The coding strand of the DNA has the same sequence as the RNA transcript (except for T in the DNA and U in the RNA). Hence, for example, the start codon for transcription is 5'-ATG in the coding strand of the DNA and 5'-AUG in the mRNA. By convention, gene, promoter, and regulatory sequences in DNA are written as they appear in the coding strand. 

二、問答題1. Explain with words, diagrams, or structures why lactate accumulates in the blood during bursts of very vigorous exercise (such as a 100-meter dash). 

2. Briefly describe the relationship of the pyruvate dehydrogenase complex reaction to glycolysis and the citric acid cycle. 

三、翻譯題The template strand of the DNA is copied during transcription, and its sequence is the complement of the RNA transcript. The coding strand of the DNA has the same sequence as the RNA transcript (except for T in the DNA and U in the RNA). Hence, for example, the start codon for transcription is 5'-ATG in the coding strand of the DNA and 5'-AUG in the mRNA. By convention, gene, promoter, and regulatory sequences in DNA are written as they appear in the coding strand. 

1. Transcription start site

2. Alternative splicing

3. DNA dependent RNA polymerase

4. Frame shift mutation

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