5. 三角測定

二、申論題(共 75 分)1. 分層抽樣(stratified sampling)和叢集抽樣(cluster sampling)都是社會科學研究的常見抽樣方式。請描述這兩種抽樣的實施方式並說明優缺點。(15 分)若有一研究要針對新北市國中學生進行問卷調查，請各舉一個分層抽樣和叢集抽樣的例子。(10 分)

(a)清晰地簡述此項數位科技如何成為貧窮相關「痛點」之解方。(15 分) (註：評分重點不在於技術是否已經存在或是可行性多高，您可以「發想」一項新技術並著重其可應用性。因此(1)清楚地描述「痛點」之問題所在與(2)簡介創新技術及(3)此創新實施如何解決問題的「邏輯性」是評分要件)

(b)如何使用質化方法來探究導入此項數位科技創新技術的影響？回答應包含研究樣本選取、研究工具選擇、資料蒐集方法、預計使用的分析方法...等。(35 分)

II.Short Questions (40%) 1. A nonapeptide was determined to have the following amino acidcomposition: (Lys)2, (Gly)2, (Phe)2, His, Leu, Met. The native peptidewas incubated with 1-fluoro-2, 4-dinitrobenzene (FDNB) and thenhydrolyzed 2,4-dinitrophenylhistidine was identified by HPLC. Whenthe native peptide was exposed to cyanogen bromide (CNBr), anoctapeptide and free glycine were recovered. Incubation of thenative peptide with trypsin gave a pentapeptide, a tripeptide, andfree Lys. 2,4-Dinitrophenyl- histidine was recovered from thepentapeptide, and 2,4-dinitrophenylphenylalanine was recoveredfrom the tripeptide. Digestion with the enzyme pepsin produced adipeptide, a tripeptide, and a tetrapeptide. The tetrapeptide wascomposed of (Lys)2, Phe, and Gly. What is the native sequence?(10%)

2. The following data were obtained in a study of an enzyme known tofollow Michaelis- Menten kinetics: (3%)The Km for this enzyme is approximately:

(1) Excitation of the chlorophyll a molecule at the reaction center

(2) Replacement of the electron in the reaction center chlorophyll

(3) Light excitation of antenna chlorophyll molecule

(4) Passage of excited electron to electron-transfer chain(5) Exit on transfer to neighboring chlorophyll

4. Yeast can metabolize D-mannose to ethanol and CO2. In addition tothe glycolytic enzymes, the only other enzyme needed isphosphomannose isomerase, which converts mannose 6-phosphateto fructose 6-phosphate. If mannose is converted to ethanol and CO2 by the most direct pathway, which of the compounds and cofactorsin this list are involved? (8%)(A) Lactate (B) Acetaldehyde (C) Acetyl-CoA (D) FAD(E) Glucose 6-phosphate (F) Fructose 1-phosphate (G) Pyruvate(H) Lipoic acid ( I) Thiamine pyrophosphate (J) Dihydroxyacetonephosphate

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