108年 - 112 國立清華大學碩士班考試入學試題_生命科學院甲組：生物化學#118786

選擇題 (20)

1. In a ______,the carbohydrate is responsible for most of the mass； in a ______, protein is responsible for most of the mass. (A) glycoprotein； proteoglycan (B) proteoglycan； glycoprotein (C) N-linked glycoprotein； O-linked glycoprotein (D) O-linked glycoprotein； N-linked glycoprotein (E) N-linked proteoglycan； O-linked glycoprotein proteoglycan the

2. Which of the following must be true if the steady state assumption is to be used? (A)[E]_T=[ES], (B)(k₂-k₁)/k₁=1, (C)k₁[E][S]=k₂[ES], (D)k₁[E][S]=k₂[ES]-k₁[ES], (E) d[ES] / dt = 0.

3. What amino acid performs the nucleophilic attack during the chymotrypsin mechanism? (A) Ser, (B) His, (C) Lys, (D) Cys, (E) Thr.

4. The ability for an enzymme to change its shape upon substrate binding represents the concept of______. (A) lock and key, (B) induced fit, (C) proximity and orientation effects, (D) covalent catalysis, (E) none of the above.

5. How does CO₂affect hemoglobin-oxygen binding? (A) CO₂binds directly to the oxygen binding site, displacing oxygen and thus promoting the deoxy state, (B) CO₂ displaces BPG, thus promoting the oxy state, (C) CO₂ is converted to bicarbonate and Ht which promotes the oxy state, (D) CO₂ is converted to bicarbonate and Ht which promotes the deoxy state, (E) None of the above.

6. Which complex reduces molecular oxygen? (A) Complex I, (B) Complex II, (C) Complex III, (D) UQH/UQH2 pool, (E) Complex IV.

7. Electrostatic interactions among amino acid residues on proteins may be damped out by high concentrations of: (A) Water, (B) Organic solvents, (C) Salts, (D) Heat, (E) All of the above.

8. Nearly all of the calcium ions in muscle are sequestered inside vesicles called: (A) Mitochondria, (B) Sarcoplasmic reticulum, (C) Endoplasmic reticulum, (D) Golgi, (E) Secretory vesicles.

9. The most significant factor in contributing protein folding between tightly packed amino acid side chains in the interior of the protein is: (A) Hydrogen bonds, (B) Electrostatic interactions, (C) Covalent ester bonds, (D) Van der Waals interactions, (E) All are true.

10. If an enzyme has a Vmax of 15 mM/min, what is the velocity if the substrate is present at 1/5 of the Km? (A) 12 mM/min, (B) 6 mM/min, (C) 3 mM/min, (D) 2.5 mM/min, (E) cannot be determined from given information.

11. Which transport process is best characterized by the Hanes Woolf plot as shown at right? ([S] is the solute concentration. V is the transport rate.)

(A) Passive diffusion, (B) Active transport, (C) Facilitated diffusion, (D) Active transport and facilitated diffusion, (E) All of the above.

12. Which of the following residue prefers to be at the membrane-water interface? (A) Gly, (B) Trp, (C) Leu, (D) Arg, (E) Gin.

13. Which of the following transport process requires energy input? (A) Facilitated diffusion, (B) Passive diffusion, (C) Secondary active transport, (D) All of the above, (E) None of the above.

14. Which of the following does not reduce Cu(II) to Cu(I), but does react with Cu(II) to produce reddish Cu₂O after invertase treatment? (A) Sucrose, (B) Glucosamine, (C) Maltose, (D) Hyaluronic acid, (E) Chitin.

15. Which of the following statement about biological membranes is TRUE? (A) Plasma membranes are asymmetric. The outer leaflet is enriched in phosphatidylserine, whereas the inner leaflet is enriched in phosphatidylcholine. (B) Membrane asymmetry is maintained predominantly by scramblase. (C) Cerebrosides are mostly found in the inner leaflet of plasma membranes. (D) Membrane curvature is controlled by cytoskeleton reorganization, and does not affected by changes in lipid composition. (E) Phospholipids with unsaturated fatty acyl chains increase membrane fluidity.

16. During the amino acid synthesis, Methionine is derived from which metabolic progenitor? (A) a-ketoglutarate, (B) Aspartate, (C) Pyruvate, (D) 3-phosphoglycerate, (E) Posphoenolpyruvate.

17. Urea contains one carbon, two nitrogens, one oxygen and 4 hydrogens. Where are the sources of the carbone and nitrogens? (A) carbamoyl phosphate and ornithine, (B) bicarbonate and aspartate, (C) carbamoyl phosphate and bicarbonate and ornithine, (D) aspartate, (E) None of the above.

18. What format of tetrahydrofolate (THF) is used for methionine biosynthesis? (A) N⁵-methyl-THF, (B) N⁵ N¹⁰-methylene-THF, (C) N¹⁰-formyl-THF, (D) N⁵-formyl-THF, (E) N⁵N¹⁰-methenyl-THF.

19. What is the active form of methionine and acts as an intracellular methyl group donor? (A) S-adenosylhomocysteine, (B) homocysteine, (C) S-adenosylmethionine, (D) 5-methylthioadenosine, (E) None of the above.

20. Which amino acid is the "pure" ketogenic amino acid? (A) tyrosine, (B) leucine, (C) histidine, (D) isoleucine, (E) cysteine.

申論題 (9)

(A) What is the pI for the following peptide: Ala-Glu-Val-Asp-Lys-Leu? (6%)

(B) What is the C-terminal amino acid in this peptide? (3%)

2. If an enzyme-catalyzed reaction with a K_M of 3.5 mM has a velocity of 5 mM/min at a substrate concentration of 0.5 mM, what is the V_max? (6 %)

3. This is a cycle of the B-oxidation of saturation fatty acid. Please calculate how many ATP can be generated in a complete oxidation of Myristic-CoA (C14:0-CoA) (if you consider one NADH produces 2,5 ATP, one FADH2 produces 1.5 ATP and one Acetyl-CoA produces 10 ATP)? (10%)

4. Please explain the mechanism of how F₁F₀-ATPase produces ATP in mitochondria. You need include the teams of proton gradient, c subunit of F₀, and a and B subunits ofF₁.(5%)

5. Please draw a potential energy diagram for the exothermic reaction A→B and explain why the enzyme-catalyzed reaction is faster than the uncatalyzed reaction. (10%)

6. Please explain why the transmembrane domains of integral membrane proteins mostly have a high secondary structure content. (5%)

7.what is the key enzyme for the biosynthesis of deoxyribonucleotides? catalytic activity of the enzyme is turned on and off in response to need for dNTPs? general, this reaction involved oxidation-reduction cycle through two re gulatory enzymes which deliver reducing equivalents. Please write names of these two enzymes? (10 %)

8.How does the completion of human genome project contribute to curr proteomic studies? (5 %)

108年 - 112 國立清華大學碩士班考試入學試題_生命科學院甲組：生物化學#118786

試卷資訊

選擇題 (20)

申論題 (9)