110年 - 110 國立臺灣大學_碩士班招生考試_生物化學暨分子生物學研究所:生化學#105531

科目：研究所、轉學考(插大)◆生化學 | 年份：110年 | 選擇題數：0 | 申論題數：28

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所屬科目：研究所、轉學考(插大)◆生化學

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申論題 (28)

__cytosine deamination

__base loss

__adenine deamination

__binds to GATC sequences

__binds to mismatch in DNA

__DNA synthesis in gaps

__seals nicks

__O⁶-methylguanine

__direct chemical reversal of pyrimidine dimer formation

__double-strand break

__excision of a lesion- containing oligonucleotide

2. In Figure below, the bacterial activator protein CAP and the Lac repressor have been placed in the four possible combinations on their binding sites in the promoter for the Lac operon. Each combination of gene regulatory proteins corresponds to a particular mixture of glucose and lactose. For each of the four combinations, indicate on the left-hand side of the figure which sugars must be present and, on the right-hand side (a-d), whether the operon is expected to be turned ON or OFF. (4%)

3. DNA synthesis on the lagging strand in E. coli is a complex process known to involve several proteins. Initiation of a new chain is catalyzed by the enzyme (a)_________ , and elongation is catalyzed by the enzyme (b)_________ Synthesis is discontinuous, yielding short segments called (c)_________ , which are eventually joined by the enzyme (d)_________ ， which requires the cofactor (e)_________ . (5%)

4. What is low-density lipoproteins (LDL)? How LDL is formed? And how it its catabolized? (8%)

5. What are ketone bodies? When and how ketone bodies are formed? (8%)

6. How many kinds of membrane proteins do you know? List them. Think about multiple ways by which proteins interact with membranes. (4%)

1) How many amino acids are there in the figure? (1%)

2) List ketogenic amino acids. (1%)

3) List strictly ketogenic amino acids. (1%)

4) List glucogenic amino acids. (1%)

5) List strictly glucogenic amino acids. (1%)

6) List ketogenic and glucogenic amino acids. (1%)

7) Which amino acid produce the most amount of acetyl-CoA per mole? How many? (2%)

8. There are different platforms of next-generation sequencing: pyrosequencing, sequencing by ligation, sequencing by synthesis, SMRT sequencing and semiconductor sequencing. Pick one and describe how it works. (4%)

1) What type of receptor do you think AR is? (2%)

2) Upon Testosterone binding to AR, what will happen next? (more than one correct answer) (3%) A) AR Dimerization B) AR degradation C) AR binding to DNA D) AR autophosphorylation of several tyrosine residues E) AR conformation change Tnstosterone JAK / STAT3 MEK / MAPK PIDK I AKT

3) Testosterone can pass through plasma membrane without a transporter. Based on this information, which molecule do you think is likely to be testosterone? (1%)

4) Which molecule do you think will be synthesized less in the presence of Aspirin? (Select one hormone structures from above) (2%)