111年 - [無官方正解]111 國立臺灣大學_碩士班招生考試：生化學#137718

1. Which of the following statement is NOT correct for the regulation of lactose (lac) operon?(A) lac operon is repressible catabolic operon.(B) lac I repressor binds to operator of lac operon in the presence of glucose.(C) Catabolite gene activator protein (CAP) is a positive control of lac operon without cAMP.(D) 1,6-allolactose functions as a true inducer.
(E) I-Z+/i+Z- diploid strain with lacZ inducibility in response to IPTG.

2. Which of the following statement is correct for the development of prophage?(A) Lambda repressor is expressed in the lysogenic bacteria cells.(B) Lambda repressor binds to OR1 to express Cro protein.(C) Lambda repressor binds to Or2 to inhibit the expression of itself.(D) Cro binds to OR1 to inhibit the expression of lambda repressor.
(E) Lysogeny is terminated by proteolysis of N protein.

3. Which of the following factor is NOT involved in the efficiency of eukaryotic transcription?(A) Histone modification(B) DNAmethylation on deoxycytidine of CpG island(C) Enhancer and silencer(D) microRNA (miRNA)
(E) Deletion of genomic information in B cells

4. Which ofthe following statement is NOT correct for the characteristics of enhancers? (A) Work when located long distances from the promoter. (B) Work when upstream or downstream from the promoter. (C) Work when only one direction is same as TATA box. (D) Work through heterologous promoters. (E) Work by binding one or more proteins.

5. Which ofthe following method is NOT for mRNA quantitation?(A) Southern blot analysis(B) Reverse transcription-quantitative polymerase chain reaction(C) cDNA microarray(D) Transcriptome analysis
(E) Next generation sequencing

6.Which of the following statement is NOT correct for the evaluation of SARS-CoV-2 infection?(A) Lower Ct (threshold cycle) value represents the higher titer of the virus.(B) Genomic RNA should be converted to cDNA before polymerase chain reaction for the quantitation of virus titers.(C) Identification of SARS-CoV-2 variant and sequences is critical to judge the cross-contamination.(D) Amicron variant contains more mutations compared to delta variant.
(E) mRNA of the nucleocapsid protein is currently used for the vaccination.

7. Which of the following statement is NOT correct for the regulation of trp operon?(A) trp operon is repressible anabolic operon.(B)In the presence of high [Trp], the attenuated mRNA of trp operon is expressed.(C)In the presence of low [Trp], trp mRNA is synthesized.(D) The formation of mRNA stem-loop 3 and 4 causes rho-dependent termination.
(E) Low level of [Trp-tRNAP^trp] causes the formation of mRNA stem-loop 2 and 3.

8.Which of the following transcription regulatory protein contains zinc finger binding motif?(A) lac repressor(B) Homeo box protein(C) Transcription factor TFIIIA(D) Fos and Jun
(E) c-myc

9. Which enzyme is involved in the ER oligosaccharides processing?(A) Oligosaccharide:protein transferase(B) N-acetyl glucosamintransferase(C) Galactose transferase(D) Fucosyltransferase
(E) Mannosidase

10. Which description about proteogłycan glycosylation is correct?(A) The posttranslational modification for directing glycoprotein transfer from ER to lysosome(B) N-acetylglucosamin Asn linkage(C) -Ser-X-Gly-Gly is the attachment site(D) N-acetylgalactosamin Asn linkage
(E) Containing Gal-Gal-Xyl trisaccharides core

11. Which is correct about the integrin connecting to intermediate filament and further transduce the mechano-sensing to the nucleus envelop? (A) Bind to laminin (B) α3ẞ1 (C) абẞ1 (D) α6ẞ4 (E) also called desmosome

12. Which following peptide can bind to a5ẞ1 integrin?(A) ARADKAA(B) ARGESAA(C) AYIGSRAA(D) AYRGDAA
(E) AYRAEKAA

13. Which is a tumor supressor protein?(A) Ras(B) Rb(C) P53(D) Myc
(E) Raf

14. Which following glycosaminoglycans can be found in the proteoglycan protein core?(A) heparan sulfate(B) dermatan sulfate(C) chondroitin sulfate(D) heparin
(E) hyaluronic acid

15. Which protein is a component for basal membrane?(A) type IV collagen(B) laminin(C) perlecan(D) type V collaben
(E) fibronectin

16. Which following protein can bind to proteolytic Laminin fragments?(A) Type IV collegen(B) a2aß3(C) a3B1(D) heparan sulfate
(E) α6ẞ4

17. Which enzyme in the cholesterol synthesis process is targeted by the cholesterol-lowering drug, statins?(A) Thiolase(B) Phosphomevalonate kinase(C)△²⁴-Reductase(D) Squalene synthase
(E) HMG-CoA reductase

18. What does "R"stand for in the terminology of restriction enzyme EcoRI?(A) Genus(B) The order of discovery(C) A strain designation(D) Species
(E) Bacterial source

19. Which cloning vector has the largest cloning capacities?(A) Plasmid(B) YAC(C) BAC(D) Cosmids
(E) Lambda vector

20. Which lipoprotein described below is the precursor of IDL?(A) Chylomicrons(B) HDL(C) VLDL(D) LDL
(E) HDL

1. Explain why a certain protein has an apparent molecular mass of 90 kD when determined by gel filtration and 60 kD when determined by SDS-PAGE in the presence or absence of 2-mercaptoethanol. Which molecular mass determination is more accurate? (4%)

a. What is the maximum number of peptides you can expect if you cleave the polypeptide with cyanogen bromides? (2%)

b. What is the maximum number of peptides you can expect if you cleave the polypeptide with chymotrypsin? (2%)

3. Two polypeptides, A and B, have similar tertiary structures, but A normally exists as a monomer, whereas B exists as a tetramer, B₄. What differences might be expected in the amino acid composition of A versus B? (4%)

a. In the amino acid sequence above, where would you predict that bends or ẞ turns would occur? (2%)

b. Where might intrachain disulfide cross-linkages be formed? (2%)

5. Match each amino acid in the left-hand column with the appropriate side-chain type in the right-hand column. (4%)
(a) Leu (1) nonpolar aliphatic
(b) Glu (2) acidic
(c) Cys (3) nonpolar aromatic
(d) Trp (4) sulfur-containing

6.What is the oxidative phosphorylation and its importance in glucose metabolism? (4%)

7. What are the molecular mechanisms for insulin to regulate the activities of phosphorylase and glycogen synthase? (4%)

8. How does glucagon regulate glycolysis and gluconeogenesis? (4%)

9. What is the important role of liver in heme catabolism and bilirubin secretion? (4%)

a. What is the disease caused by inherited mutation of the HGPRT? (2%)

b. What is the size of the HGPRT-cDNA? (2%)

c.What is the size of the HGPRT protein? (2%)

d. Which of the following plasmids does contain the HGPRT-cDNA? (2%)
(A) Plasmid#1 (B) Plasmid#2(C) Plasmid#3(D) Plasmid#4(E) Plasmids#1 and 2 (F) Plasmids#1 and 3
(G) Plasmids#2 and 4 (H) Plasmids#3 and 4 (I) All of them

e. Which of the following plasmids is the pCMV/HGPRT-cDNA? (2%)
(A) Plasmid#1(B) Plasmid#2 (C) Plasmid#3(D) Plasmid#4 (E) Plasmids#1 and 2 (F) Plasmids#1 and 3
(G) Plasmids#2 and 4 (H) Plasmids#3 and 4 (I) All of them

f. Please give one possible reason why the two experiments have different results. (4%)

g. What is a useful alternative method to determine which plasmids contain the HGPRT-cDNA? (2%)