複選題

11. Which of the following factor(s) can bind Pab1p, a poly[A]-binding protein?
(A) eIF1
(B) eIF1A
(C) eIF4A
(D) eIF4E
(E) eIF4G

7. The size of a gene in the genome is 3kb. What is the expected size of the polypeptide product encoded from the 3kb sequence? It appears as a single 60kD band on a SDS-PAGE gel. What could be the explanation for the difference? (A) 110kD； presence of introns (B) 110kD； protein degradation (C) 55kD； post-translational modifications (D) 55kD； amino acid composition bias (E) 30kD； dimer formation

複選題8. Which of the following factor(s) promote dissociation of 70S ribosome to 50S and 30S? (A) IF-2 (B) IF-3 (C) EF-Tu (D) EF-G (E) RRF

9. Which of the following factor(s) mediates the binding of the Shine-Dalgarno sequence with the complementary sequence of the 16S rRNA? (A) IF-2 (B) IF-3 (C) EF-Tu (D) EF-G (E) RRF

10. Which of the following descriptions is NOT correct for comparison of protein translation between eukaryotes and bacteria? (A) Bacterial translation begins with methionine (B) Bacterial translation requires Shine-Dalgarno sequence to show ribosomes where to start (C) Eukaryotic mRNA has caps at 5'end (D) Eukaryotic initiating tRNA is not same as tRNA for interior (E) Eukaryotic translation requires Kozak sequence to show ribosomes where to start

12. Which of the following factor(s) has an RNA helicase activity in translation initiation complex? (A) eIF1 (B) eIF1A (C) eIF4A (D) eIF4E (E) eIF4G

複選題13. Which of the following factor(s) are involved in dissociating improper complexes between 40S subunits and mRNA? (A) eIF1 (B) eIF1A (C) eIF4A (D) eIF4E (E) eIF4G

14. Which of the following descriptions is NOT correct for the peptidyl transferase in ribosome? (A) The peptidyl transferase resides on the 50S ribosomal particle (B) The peptidyl transferase activity is GTP-dependent (C) Minimum components necessary for its activity are 23S rRNA and proteins L2 and L3 (D) 23S rRNA is at the catalytic center of peptidyl transferase (E) puromycin can be its substrate

複選題15. Which of the following description about histone is (are) correct? (A) The molecular mass of Histone H4 is bigger than the other types of histones. (B) Histones H2A/H2B and H3/H4 dimerize through the non-covalent interaction of a highly conserved structural motif found in each core histone. (C) The histone H3 is extreme highly conserved from pea to cow, which differs by only two residues. (D) Histones H1 and H5 bind as monomers to nucleosomes and internucleosomal linker DNA to facilitate higher-order DNA compaction. (E) The heterogeneity of histone proteins is due to alternative splicing.

複選題16. Which of the following description about histones modification is (are) correct? (A) Histone acetylation specifically occurs in the nucleus. (B) The bromodomain domain of HAT(histone acetyltransferase) recognizes acetylated lysine residues on the N-terminal tails of histones. (C) The type B HAT does not contain the bromodomain. (D) The SANT domain of the type A HAT binds to DNA instead of histone. (E) HDAC deacetylates lysine on the N-terminal tail of core histone, leading to activation of gene transcription.

複選題17. Which of the following description about RNA polymerase II (Pol II) is (are) correct? (A) Pol II is a multiprotein complex that transcribes DNA into precursors of mRNA and microRNA. (B) The C-terminal domain (CTD) of the RNA polymerase binding protein (Rpb1) subunit of Pol II interacts with the splicing factor and stimulates splicing of substrates in organisms that use exon definition. (C) The facilitates chromatin transcription (FACT) complex is a histone chaperone comprising Spt16 and SSRP1, which is involved in transcription elongation by RNA polymerases II. (D) The CTD of the Rbpl subunit is involved in capping and splicing but not in polyadenylation. (E) The cotranscriptional cleavage (CoTC) element is essential for transcription termination.