複選題

16. Bromodeoxyuridine (BrdU) is a synthetic nucleoside that is an analog of
thymidine and therefore can be used to label newly synthesized DNA. Lily has
treated cells with BrdU transiently for 2 hours, and then immediately uses the cell
sorting machine to collect cells with different DNA content. Which fraction of the
cell do you expect to detect BrdU signal?
(A)A
(B)B
(C)C
(D)D

(E)C+D

1. Which of the following is the correct order of chromatin folding?(A)30 nM fiber formation, nucleosome formation, radial loop formation(B)radial loop structure, 30 nM fiber formation, nucleosome formation(C)nucleosome formation, 30 nM fiber formation, radial loop structure(D)30 nM fiber formation, radial loop formation, nucleosome formation(E)nucleosome formation, radial loop formation, nucleosome formation

2. Which of the following descriptions of chromatin remodeling is NOT correct?(A)Remodeling requires ATP for energy(B)BRG1 associates with BAFS(C)BRG1 is an ATPase(D)SWI/SNF binds to DNA through SANT domain(E)All SWI/SNF, ISWI, NuRD and INO80 family proteins make the DNA moreaccessible

3. The binding of which of the following snRNPs to spliceosome requires ATP?(A)U1(B)U2(C)U4(D)U5(E)U6

4. The catalytic center of the spliceosome appears to include(A)Mg2+(B)U2 and U6 snRNP(C)the branch point region of the intron.(D)Mg2+ and the branch point region of the intron.(E)Mg2+, U2, and U6 snRNP, and the branch point region of the intron.

5. Which of the following is NOT the possible action for miRNA?(A)targets viral DNAs(B)mRNA degradation(C)stimulate translation(D)translation initiation blockade(E)translation elongation blockade

6. Which of the following is responsible for the binding between the 30S ribosomal subunit and the initiation site of a message? (A)Kozak sequence (B)Shine-Dalgarno sequence (C)TATA Box sequence (D)Internal Ribosome Entry sequence (E)Ribosome Exit sequence

7. Which of the following initiation factor is a target for translation control byheme-controlled repressor (HCR) in heme-starved reticulocytes?(A)eIF1(B)eIF2(C)eIF3(D)eIF4F(E)eIF6

8. Which of the following factor is a guanine nucleotide exchange factor (GEF)involved in protein translation?(A)eIF5A(B)eIF5B(C)EF-Tu(D)EF-Ts(E)EF-G

9. Which of the following description is NOT correct for translocation intranslational elongation during protein synthesis?(A)GTP and EF-G facilitate translocation.(B)Translocation activity appears to be inherent in the ribosome.(C)Translocation activity can be expressed without EF-G and GTP in vitro.(D)GTP hydrolysis occurs after translocation.(E)New round of elongation cannot occur if EF-G is not released from theribosome.

10. Which of the following factor does NOT bind to the A site of ribosome?(A)IF2/tRNA(B)EF-Tu/tRNA(C)EF-G(D)RF3/RF1(E)RRF

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