複選題

20. E. coli DNA polymerases IV and V are:
(A) RNA-dependent DNA polymerases.
(B) for restarting replication.
(C) for priming replication.
(D) error-prone polymerases.
(E) All of these are correct.

21. Although E. coli DNA polymerase I is not a replicase, it participates in DNA replication. What is its function? (A) It synthesizes Okazaki fragments. (B) It synthesizes an RNA primer. (C) It unwinds the double helix. (D) It uses nick translation to replace the RNA primer with DNA. (E) It connects the 3' end of one Okazaki fragment with the 5' end of another.

22. Which proteins function in the resolution of Holliday junctions? (A) The Ruv complex (B) RecA (C) Zip proteins (D) The RecBCD complex (E) DNA polymerase I

23. The human disease xeroderma pigmentosum results from mutations in genes involved with: (A) nucleotide excision repair. (B) photoreactivation. (C) base excision repair. (D) mismatch repair. (E) recombination-repair.

24. Which of the following is not true concerning immunoglobulin gene recombination signal sequences? (A) There are conserved heptamers. (B) There are conserved nonamers. (C) Recombination occurs between a 12 bp signal and a 23 bp signal. (D) The conserved sequences are separated by a non-conserved sequence of either a12 bp or a 23 bp sequence. (E) Recombination only occurs between two heptamers.

25. All of the following are proteins within the core nucleosome particle EXCEPT: (A) H1 (B) H2A (C) H2B (D) H3 (E) H4

複選題26. Which ones of the following descriptions about RNA editing are correct? (A) trypanosome kinetoplast RNA editing occurs in both 3' 5'and 5'3' directions (B) mRNA must be edited before being translated (C) Both ADAR and CDAR could mediate nucleotide deamination (D) All 4 ribonucleotides (A, U, C, G) in RNA could be edited (E) one or more gRNAs to hybridize the edited region.

27. Which of the following splicing factors base-pair with pre-mRNA? (A) U6 (B) U5 (C) U4 (D) U2 (E) U1

28. The synthesis of mRNA's that encode the proteins of eukaryotic RNA polymerase occurs in the: (A) cytoplasm (B) nuclear envelope (C) nucleolus (D) euchromatin (E) heterochromatin

複選題29. Which of the following description of RNA processing events is correct? (A) Cap is a modified 7-methylguanosine that 5'-3' links to the 5'terminus of RNA. (B) RNA polyadenylation only happens in nucleus. (C) RNA cap can be labeled with [B,y-32P]ATP but not with [y-32P]ΑΤΡ. (D) RNA polymerase II transcription of a gene usually stops at the polyadenylation site. (E) Once an mRNA loses its poly（A） tail, it is imported back into the nucleus for replenishing poly（A）tail.

30. Which of the following snRNP is mismatched with its function? (A) U1: base pairs with 5' splice site of mRNA. (B) U2: base pairs with the conserved sequence at splicing branch point. (C) U4: base pairs with 3' splice site of mRNA. (D) U5: associates with last nucleotide in one exon and the first nucleotide in the next exon. (E) U6: base pairs with 5' end of the intron.

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