複選題
65. X uses a Y to link cofactor, Z. Which one of the followings is NOT true?
(A) X: Pyruvate dehydrogenase； Y: lysine residue； Z: lipoamide
(B) X: Aconitase； Y: cysteine residue； Z: iron-sulfur center
(C) X: Pyruvate carboxylase； Y: lysine residue； Z: biotin
(D) X: Acyl carrier protein； Y: cysteine residue； Z: pantothenate
(E) X: Aminotransferase； Y: lysine residue； Z: pyridoxal phosphate

61. Enzymes are classified into 7 different major classes. Which enzyme class does the following reaction belong to? Pyruvate + H+ → acetaldehyde + CO2 (A) Oxidoreductases (B) Transferases (C) Hydrolases (D) Lyases (E) Ligases

62. Which of the following statements about the enzyme regulation by covalent-modification is NOT true? (A) Phosphorylation means the transfer of phosphate group from ATP to proteins. (B) Adenylylation means the transfer of an adenylate moiety from ATP to proteins. (C) ADP-ribosylation means the transfer of an ADP-ribosyl moiety from ATP to proteins. (D) Acetylation means the transfer of an acetyl group from acetyl-CoA to proteins. (E) Phosphatases can remove phosphate group from proteins.

複選題63. Which of following statements regarding glycogen metabolism is TRUE? (A) Glycogen degradation starts from reducing end to non-reducing end. (B) Amylo-(1,4 → 1,6)-transglycosylase is used in glycogen synthesis. (C) Glucose-1-phosphate is the substrate of glycogen synthase. (D) The regulation mechanism of glycogen phosphorylase b includes both allosteric control and covalent-modification. (E) Protein kinase C triggers the glycogen breakdown cascade.

64. Which of the followings is NOT required for the transportation of NADH from cytosol into mitochondria? (A) malate (B) α-ketoglutarate (C) citrate (D) oxaloacetate (E) aspartate

66. Which of following statements is NOT true regarding the lysozyme’s Phillips mechanism? (A) Glu 35 acts as both “general acid” and “general base” catalysis. (B) The active site of lysozyme can take six residues of monosaccharides. (C) The D ring of monosaccharide is distorted into boat form conformation. (D) The oxonium ion transition state is stabilized by Asp 52. (E) The optimal pH of this reaction is between 4 and 6.

67. Enzyme X follows Michaelis-Menten kinetics. As shown in the figure, its value of Vmax and Km can also be obtained by using Eadie-Hofstee plot. The initial reaction rate is v0 with the substrate concentration [S]. What is the intercept on v0/[S] axis?(A) Vmax/Km (B) 1/Km (C) Km (D) Vmax (E) 1/Vmax

68. Among 9 different molecules below, X of them can cleave disulfide bond, and Y of them can react with the amino group of L-amino acids. Therefore, X + Y =__________ .cyanogen bromide； iodoacetate； phenylisothiocyanate； β-mercaptoethanol； performic acid； 9 -fluorenylmethoxycarbonyl chloride； dithiothreitol；； guanidinium hydrochloride； urea. (A) 2 (B) 3 (C) 4 (D) 5 (E) 6

69. How many of the followings are ketogenic amino acid in human? Asp； Phe； Arg； Val； His； Thr； Lys. (A) 2 (B) 3 (C) 4 (D) 5 (E) 6

70. How many items of the followings have the activity of proof-reading to reduce the error rate in biosynthesis?DNA polymerase； RNA polymerase II； amino-acyl tRNA synthetase； Initiation Factor-1； polyketide synthase； Elongation Factor-Tu； Klenow fragment. (A) 2 (B) 3 (C) 4 (D) 5 (E) 6

【已刪除】71. Which one of followings is NOT used for thymine dimer repair? (A) Daughter strand gap repair (B) Base excision repair (C) Nucleotide excision repair (D) Photolyase (E) DNA polymerase Eta (Pol )