11. Which of the following is the primase that synthesizes primers during DNA
replication in E. coli?

(A)RNA polymerase

(B)HU protein

(C)DnaA

(D)DnaB

(E)DnaG

12. Which of the following subunits of DNA polymerase III holoenzyme is referred to as the "sliding clamp"? (A) α (B) β (C)γ (D)x(E) θ

13. Which of the following is NOT part of the E. coli primosome?(A)DnaA(B)DnaB(C)DnaG(D)TerE(E)oriC

14. Chi sites stimulate which of the following recombination pathways?(A) λ Red(B) λ Int(C)E. coli RecBCD(D)E. coli RecE(E)E. coli RecF

15. Hybrid dysgenesis refers to the fact that in Drosophila a cross between a P male and an M female produce offspring that are (A)blind. (B)dead. (C)hairless. (D)sterile. (E)wingless.

複選題16. Bromodeoxyuridine (BrdU) is a synthetic nucleoside that is an analog ofthymidine and therefore can be used to label newly synthesized DNA. Lily hastreated cells with BrdU transiently for 2 hours, and then immediately uses the cellsorting machine to collect cells with different DNA content. Which fraction of thecell do you expect to detect BrdU signal?(A)A(B)B(C)C(D)D(E)C+D

17. A typical Tag DNA polymerase makes 1 nucleotide mistake in every 10^5nucleotide copies. However, the error rate of DNA replication in a human cell isabout 1/10^9. Which of the following is NOT the reason for this high fidelity?(A)DNA polymerase can proofread(B)DNA polymerase is still active at 95 degree in Celsius.(C)DNA polymerase can couple with the mismatch repair(D)DNA methylation serves as a template for correcting mistakes(E)DNA polymerization occurs only from 5' to 3'

18. Which of the following pairs of codons might you expect to be read by the same tRNA as a result of wobble?(A)CUU and UUU(B)GAU and GAA(C)CAC and CAU(D)AAU and AGU(E)UAG and UAA

複選題19. Which of the following RNA may block translation of targeted proteins?(A)miRNA(B)mRNA(C)siRNA(D)Inc RNA(E)dsRNA

20. Which of the following is a stop codon?(A)AUG(B)UGG(C)GAG(D)CGA(E)UAG

1. When a repressor binds to the operator site on the DNA it normally______.(A) terminates transcription that has already been initiated.(B) inhibits the initiation of transcription.(C) reduces the rate of transcription.(D) has no effect on transcription.

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