17. Which description is correct?

(A) DNA polymerase can synthesize DNA in both the 3'5' and 5'→3' directions.

(B) DNA replication in E. coli is initiated at multiple sites.

(C) DNA replication in eukaryotes is initiated at a unique site.

(D) DNA ligase cannot link two single-stranded DNA fragments.

18. There are three types of RNA polymerases in eukaryotes. Which description is NOT correct?(A) RNA polymerase I synthesize 5S rRNA.(B) RNA polymerase II synthesizes mRNA.(C) RNA polymerase II synthesizes snRNA.(D) RNA polymerase III synthesizes tRNA.

19. Functions of RNA polymerase includes____________.(A) Searching for promoter sites.(B) Unwinding short stretches of DNA.(C) Detecting termination signals.(D) All of the above.

20. Most genes in higher eukaryotes are composed of exons and introns. The introns must be excised in a process called RNA splicing. Which description is correct? (A) Yeast has no RNA splicing. (B) The consensus sequence at the 5' splice site is AG. (C) The 5' and 3' splice sites and the branch site are essential for RNA splicing. (D) Six snRNAs-designated U1, U2, U3, U4, U5, U6-are essential for RNA splicing.

21. Which description is not correct about tRNA molecules?(A) All are single-stranded RNAs.(B) About half of the nucleotides are base-paired.(C) They contain a lot of unusual bases.(D) The 3' terminal residues are usually CAA.

22. Ribosome biogenesis is the process of making ribosomes, it take place in the______.(A) Nucleoplasm.(B) Nucleolus.(C) Cytoplasm.(D) None of the above.

23. The lac operon is a common regulatory unit in prokaryotes. Which description is NOT correct?(A) When glucose is present, the lac operon is turned off.(B) When lactose is absent, the lac operon is turned off.(C) When lactose is present, the lac operon is turned on.(D) IPTG induces the lac operon by binding to the promoter.

24. Gene expression can be controlled at post-transcriptional levels. Which description is NOT correct?(A) The 5' capping protects the mRNA from degradation by exonuclease.(B) The mRNA splicing occurs only when transcription is fully completed.(C) The 3' polyadenylation has effects on mRNA stability.(D) RNA interference functions in the cytoplasm.

25. Select the correct statement about enhancers.(A) They are proteins that promote transcription of RNA.(B) They stimulate the binding of repressor to DNA.(C) They bind protein factors and stimulate transcription.(D) They are nonpromoter protein elements.

26. Which of the following techniques is most useful in determining if RNA polymerase has initiated transcription from the lac DNA template? (A) Southern analysis (B) DNA fingerprinting (C) run-off transcription assay (D) DNA sequencing

27. Which of the following is NOT present in the core RNA polymerase?(A)β(B) β'(C) σ(D) None of the above.

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