試題詳解

19. Marry wants to clone a gene from a cDNA library. She designed a pair of primers and performed PCR, but she surprisingly found two PCR products. She then sequenced it, and found that two PCR products are mostly matching to the target gene except for having an intron in one of them. What could be the most possible reason?
(A) incomplete splicing of the gene
(B) trans-splicing of the gene
(C) point mutation in primers
(D) low Tm for PCR reaction
(E) wrong buffer condition