25. What main compound is transported from the cytosol to the mitochondria via malate-aspartate shuttle?
(A) NADH
(B) NH4 +
(C) FADH2
(D) O2
(E) oxaloacetate

21. In order to perform PCR, the following reagents must be included ________. (A) DNA fragment, primers flanking the region of interest, dNTPs, ddNTPS, DNA Polymerase (B) DNA fragment, primers flanking the region of interest, dNTPs, DNA Polymerase (C) DNA fragment, one primer, dNTPs, DNA Polymerase, DNA ligase (D) DNA fragment, primers flanking the region of interest, dNTPs, DNA Ligase (E) none of the above

22. The coding sequence of DNA is 5’-CGCTATAGCGTTT-3’. Which of the following sequences is its RNA transcript (mRNA)? (A) 3’-CGCTATAGCGTTT-5’ (B) 5’-CGCUAUAGCGUUU-3’ (C) 3’-GCGATATCGCAAA-5’ (D) 5’-UUUCGCUAUAGCG-3’ (E) 5’-CGCTATAGCGTTT-3’

23. Gout is caused by ___ over degradation results in accumulation of uric acid. (A) purine (B) pyrimidine (C) fatty acid (D) glucose (E) amino acid

24. Which of the following peptides has the largest molar extinction coefficient for UV absorption at 280 nm? (A) Tyr-Glu-Gly-Lys (B) Phe-Arg-His-Phe (C) Gln-His-Cys-Ala (D) Val-Asp-Met-Trp (E) Ile-Lys-Asn-Ser

26. What enzyme can generate citrulline? (A) NO synthase (B) citrate dehydrogenase (C) citrate oxidase (D) citrulline synthetase (E) citrate peroxidase

27. The Tang Prize in Biopharmaceutical Science was awarded to Dr. Feng Zhang for ________. (A) cancer immunotherapy (B) vesicular transport (C) mitochondrial fusion and fission (D) discovery of nerve growth factor (E) the development of CRISPR/Cas9 as a genome editing platform

28. What amino acid residue is required for the protein region to interact with Src homology 3 (SH3) domain? (A) serine (B) proline (C) lysine (D) arginine (E) leucine

29. Molecular chaperones assist proteins in the formation of ________. (A) aggregates (B) 3° structure (C) amide bonds (D) 1° structure (E) none of the above

複選題30. Supercoiled DNA can be relaxed by ________. (A) catenation (B) intercalation (C) nicking one strand (D) hybridization (E) denaturation

Ⅱ.【單選題】每題 2 分，共計 120 分。答錯 1 題倒扣 0.5 分，倒扣至本大題零分為止，未作答，不給分亦不扣分。 31~60 題為普通生物學，61~90 題為生化概論。 31. Which of the following statements about transporters is incorrect? (A) Proton pump is an electro-genic pump. (B) Transport of sodium ions and potassium ions through sodium-potassium pump is an active transport. (C) The membrane potential acts as an energy source that affects the traffic of charged substances across the membrane. (D) Facilitated diffusion is a diffusion of solutes against their gradients. (E) The transport of glucose via glucose transporter is affected by the temperature.