25. Which of the following is the key bacteriophage X regulatory protein that acts as an activator and establishes lysogeny upon infection of a new host?
(A) CI.
(B) CII.
(C) CIII.
(D) Cro.

(E) Q

26. Which of the following DNA binding domains or motifs is found in most bacterial regulatory proteins? (A) Zinc-finger domain. (B) Basic leucine zipper motif. (C) AT-hook motif. (D) Helix-turn-helix motif. (E) Helix-loop-helix motif.

27. High throughput sequencing technologies become powerful tools to study molecular biology on a genome-wide scale. Which of the following sequencing techniques is used to assess the translational status? (A) RNA binding protein immunoprecipitation-sequencing (RIP-Seq). (B) Chromatin immunoprecipitation-sequencing (ChIP-Seq). (C) Global run-on sequencing (GRO-Seq). (D) 4-thiouridine sequencing (4sU-Seg). (E) Ribosome sequencing (Ribo-Seq).

複選題28. Which of the following statements is correct regarding riboswitches? (A) Most riboswitches are trans-regulatory elements. (B) Riboswitches can regulate gene expression at both transcriptional and translational levels. (C) The riboswitch region that binds the small-molecule ligand is called the receptor. (D) Riboswitches are typically found downstream of genes involved in the synthesis of the metabolite ligand recognized by the riboswitch. (E) Riboswitches can mediate rho-dependent transcription termination.

複選題29. Which of the following statements is correct regarding clustered regularly interspaced short palindromic repeats (CRISPR)? (A) CRISPR is found in the eukaryotic genome. (B) A CRISPR locus consists of 2 major components: repeated and spacer sequences. (C) The spacer sequences are acquired from infecting viruses. (D) Cas1 and Cas2 are involved in processing of CRISPR RNA (crRNA). (E) The CRISPR/Cas6 system is currently the most widely utilized tool for genome

30. Which of the following statements is correct regarding small RNA-mediated silencing pathways in eukaryotes? (A) Piwi-interaction RNAs (piRNAs) are expressed predominantly in vegetative cells. (B) Both small interfering RNAs (siRNAs) and microRNAs (miRNAs) are generated from longer RNA molecules by Dicer. (C) The major component of the RNA-induced silencing complex is called Drosha. (D) The size of small RNAs is typically in the range of 30-40 nucleotides. (E) The miRNA is typically highly specific with only one mRNA target. editing.

複選題31. Which of the following statements are correct regarding the central dogma of molecular biology, as firstly stated by Francis Crick in 1957? (A) The sequential information can be transferred from DNA to RNA. (B) The sequential information can be transferred from RNA to DNA. (C) The sequential information can be transferred from RNA to protein. (D) The sequential information can be transferred from protein to RNA. (E) The sequential information can be transferred from protein to protein.

複選題32. Which of the following histones form a tetramer in the first step of the assembly of a nucleosome? (A) H1. (B) H2A. (C) H2B. (D) H3. (E) H4.

複選題33. Which of the following features are correct regarding the mechanism of DNA polymerase? (A) DNA polymerase uses a single active site to catalyze the addition of any of the four deoxynucleoside triphosphates. (B) Steric constraints prevent DNA polymerase from using ribonucleoside triphosphates as substrate. (C) The thumb domain of DNA polymerase is intimately involved in catalysis. (D) Processivity is a crucial character for DNA polymerase and defines the average number of nucleotides added per template binding event. (E) Removal of incorrectly base-paired nucleotides is mediated by intrinsic endonuclease activity of DNA polymerase.

複選題34. Which of the following pairs between the type of DNA damage repair and the corresponding enzyme are correct? (A) Nucleotide excision repair: DNA glycosylase. (B) Photoreactivation: DNA photolyase. (C) Base excision repair: UvTABC endonuclease. (D) Mismatch repair: MutS and MutL. (E) Double-strand break repair: Rec A recombinase.

複選題35. Which of the following mechanistic features of RNA polymerase are correct? (A) RNA polymerase acts to make RNA copies from the whole genome. (B) RNA polymerase uses DNA as a template for RNA synthesis. (C) RNA polymerase needs a primer for initiation of RNA synthesis. (D) The error rate of RNA polymerase is lower than that of DNA polymerase. (E) Prokaryotic mRNA and non-coding RNA are synthesized by the same RNA polymerase.

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