29. In the construction of an expression vector, which of the following would you include in order to stimulate a high level of RNA synthesis?
(A) P3
(B) T7 phage promoter
(C)
(D) His region
(E) GFP

30. Which of the following cloning vectors would you use to clone an insert of size 500 kb? (A) Plasmid vector (B) Shuttle vector (C) Cosmid vector (D) Phage λ vector (E) Yeast artificial chromosome

31. Which of the following statements concerning the constitutive heterochromatin is     true    ? (A) Gene dense region (B) Centromeric location (C) High Cot value (D) Early replicating (E) None of above

32. Meiotic recombination is initiated at (A) Late interphase (B) Early to mid prophase (C) Mid to late prophase (D) The appearance of chiasmata (E)

33. A temperature sensitive mutant refers to (A) A mutation of one of anti-freeze genes (B) A mutation of one of the heat-shock genes (C) A specific type of gene mutation resulting in instability of gene product on non-permissive temperature (D) A mutation of gene resulting in unstable genome at non-permissive temperature (E) All of above

34. Walker and colleagues tested three bacteria for their ability to revert (from his to ) after UV irradiation. Results of their experiment are shown below. Which statement is     incorrect     regarding the function of UmuC? (A) umuC mutation decreases the reversion rate (B) Overexpression of UmuC increases the reversion rate (C) This result indicates that umuC is essential for faithful repair of UV-induced DNA damage. (D) Bacterium devoid of umuC activity was essentially unmutable. (E) None of above

35. Regarding DNA repair, which of the following statements is     incorrect    ? (A) DNA photolyase in E. coli is involved in breaking pyrimidine dimers formed by UV radiation. (B) Patients with Xenoderma pigmentosum are extremely sensitive to ultra violet irradiation due to defects in base excision repair. (C) Microsatellite instability is frequently found in patients with a defective mismatch repair. (D) Double-strand breaks in eukaryotes are repaired by homologous recombination and non-homologous end joining. (E) mismatch repair system of E. coli utilizes the DNA methylation to differentiate template and newly synthesized DNA strand.

36. Which of the following statement concerning the Ac-Ds of maize is     incorrect    ? (A) Ds element cannot transpose by itself. (B) Ac element cannot transpose by itself. (C) These DNA elements can induce chromosome breakage. (D) These DNA elements can induce the formation of dicentric chromosome. (E) None of above

37. Which of the following subunits of DNA polymerase III holoenzyme is referred to as the "sliding clamp"? (A) α (B) β (C) γ (D) θ (E) τ

38. Given the following cell types, which do you propose would have the highest levels of telomerase activity? (A) Monkey liver cell (B) Macrophage (C) Colon cancer cell (D) Schwann cell (E) Osteoclast

39. Which of the following is     incorrect     concerning immunoglobulin gene recombination signal sequences? (A) There is a conserved heptamer. (B) There is a conserved nonamer. (C) The conserved sequences are separated by a nonconserved sequence of either a 12 bp or a 23 bp sequence. (D) Recombination occurs between a 12 bp signal and a 23 bp signal. (E) None of above

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