3. A DNA element that binds protein factors and stimulates transcription is called _____
(A) Enhancer
(B) Promoter
(C) Suppressor
(D) Operator
(E) Terminator

4. Which of the following statement is correct regarding the sigma-factor? (A) Subregions 2.4 and 4.2 are involved in operator recognition. (B) Region 1 appears to be in preventing σ from binding to DNA by itself. (C) It determines rifampicin sensitivity or resistance. (D) Subregion 2.4 has a β-sheet domain. (E) Region 3 is the most highly conserved σ region.

5. During an experiment to study the rate of infection of bacteria with a phage, the bacterial cultures were accidentally exposed to a UV light source. Which of the following would be expected for this exposure? (A) recA gene is turned off. (B) Coprotease activity in repressor is activated. (C) Lysogenic cycle is induced. (D) Coprotease activity in RecA protein is activated. (E) SOS response is turned off.

6. Which of the following statements is not true concerning DNA replication in prokaryotes? (A) Semidiscontinuous (B) Semiconservative (C) RNA dependent. (D) There is one terminus of replication. (E) There is one origin of replication.

7. Which of the following is not a characteristic of the prokaryotic single stranded DNA binding (SSB) protein? (A) It binds more tightly to ssDNA than to dsDNA. (B) It aids helicase action. (C) It stimulates homologous DNA polymerase. (D) It keeps DNA strands from annealing. (E) It is not essential for cell replication.

8. Put the following steps of E. coli primosome assembly in the correct order. (1) DnaG binds. (2) DnaA binds to oriC at dnaA boxes. (3) DnaB binds to the open complex. (4) DnaA, ATP, and HU protein melt the DNA. (A) 2, 4, 1, 3 (B) 2, 4, 3, 1 (C) 4, 1, 2, 3 (D) 4, 2, 1, 3 (E) 3, 4, 1, 2

9. The Ds transposable element of maize cannot transpose on its own because it lacks (A) Tn3. (B) inverted repeats. (C) origin of replication. (D) direct repeats. (E) transposase.

10. Which of the following is not true concerning immunoglobulin gene recombination signal sequences? (A) There are conserved heptamers. (B) There are conserved nonamers. (C) Recombination only occurs between two heptamers. (D) The conserved sequences are separated by a nonconserved sequence of either a 12 bp or a 23 bp sequence. (E) Recombination occurs between a 12 bp signal and a 23 bp signal.

11. Which DNA segment contains the Ori sequence? (A) Segment A (B) Segment B (C) Segment C (D) Segment D

12. Assume that a lagging strand is made from the Region X, what will be the sequence of this lagging strand? (A) 5'-CCAGTTAAC-3' (B) 5'-GTTAACTGG-3' (C) 5'-GGTCAATTG-3' (D) 5'-CAATTGACC-3'

13. Which of the following best explains why the two DNA polymerase proteins that are held by the sliding clamp are oriented in the opposite directions? (A) The efficiency of replication is increased if the two DNA polymerases synthesize the template in the opposite directions (B) DNA polymerase is such a large protein complex that steric hindrance prevents both polymerases from following the replication fork (C) DNA polymerase can read only the template from 5' to 3' direction and the template strands are aligned in an anti-parallel direction (D) DNA polymerase can read only the template from 3' to 5' direction and the template strands are aligned in an anti-parallel direction (E) None of the above

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