試題詳解

4. Agrobacterium-mediated T-DNA delivery of CRISPR/Cas9 expression cassettes is an advanced tool for genome editing in plants. Which of the following descriptions about this technique is not correct?
(A) Only the T-DNA region is transferred from agrobacterium into the host plant's nuclear DNA genome.
(B) The virulence region of the Ti plasmid encodes proteins to form a membrane- spanning DNA transporter called the type IV secretion system (T4SS).
(C) CRISPR/Cas9 cleaves foreign DNA via two components, Cas9 and sgRNA.
(D) The sgRNA is a synthetic RNA containing a 5'-end with a 20-nt sequence that acts as a guide sequence to identify the target sequence accompanied by a protospacer adjacent motif (PAM) sequence.
(E) When a template is present, nonhomologous end-joining (NHEJ) can be activated and results in gene replacement or knock-in.