45. Which of the following sequence of the steps is regular for Western Blotting Assay that is widely used for detection of the specific protein in a tissue?
1. Tissues are broken down using a homogenizer or by sonication.
2. The protein is probed with an addition of the primary antibody.
3. The protein is transferred to a PVDF membrane.
4. Add secondary antibody conjugated with peroxidase.
5. The proteins of the tissues are separated using gel electrophoresis.
6. The protein is detected with an addition of a peroxidase substrate.
(A) 123456
(B) 153246
(C) 152346
(D) 154623
(E) 124653
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