58. Which of the following statements about eukaryotic RNA polymerase II is correct?
(A) The yeast RNA polymerase II is composed of 12 different protein subunits.
(B) All three eukaryotic RNA polymerases share two common protein subunits.
(C) The enzyme is usually located in the nucleolus.
(D) The enzyme is mainly responsible for the transcription of tRNA and 5S rRNA genes.
(E) Among the three eukaryotic RNA polymerases, RNA polymerase II activity is less inhibited by a-amanitin.

54. Proteins can be modified with small regulatory proteins like ubiquitins and small ubiquitin-like protein modifiers (SUMO). Which of the following descriptions about SUMO is NOT correct? (A) SUMO are a highly conserved family of proteins found in all eucaryotes. (B) SUMO are covalently ligated to lysine residues in target proteins. (C) Sumoylated proteins are primarily targeted to the proteasome for destruction. (D) Protein sumoylation is primarily a 3-enzyme conjugating system. (E) Protein sumoylation can be reversed by a small number of isopeptidases that cleave the target protein-SUMO linkages.

55. The Hsp70 molecular chaperones are a group of proteins that assist nascent protein folding. Which of the following statements about the E. coli Hsp70 is NOT correct? (A) In Hsp70-assisted folding, the chaperone binds to nascent polypeptide chains while they are still on the ribosomes. (B) The DnaJ protein binds unfolded proteins during protein synthesis and delivers them to the DnaK: ATP complex. (C) The Dnak protein recognizes exposed, extended regions of polypeptides that are rich in hydrophilic residues. (D) Interaction of DnaK with DnaJ triggers the ATPase activity of DnaK. The resulting DnaK: ADP complex forms a stable complex with the unfolded polypeptide. (E) The reaction cycle requires a nucleotide exchange protein GrpE.

56. Which of the following descriptions about pyrimidine synthesis is NOT correct? (A) The pathway commences with the conversion of bicarbonate and glutamine into carbamoyl phosphate. In mammalian cells, carbamoyl phosphate synthetases- II is the major enzyme responsible for the reaction. (B) Carbamoyl phosphate reacts with aspartate to produce carbamoyl aspartate. The enzyme aspartate transcarbamoylase catalyzes this reaction. (C) The enzyme dihydroorotase converts carbamoyl aspartate into dihydroorotate. This step does not involve the incorporation of any atoms from the other substrates. (D) Dihydroorotate is combined with 5-phosphoribosyl-1-pyrophosphate to form UMP. This step is catalyzed by orotate phosphoribosyltransferase. (E) Unlike purines, pyrimidines are not synthesized as nucleotide derivatives. The synthesis of the pyrimidine ring is completed before a ribose-5-phosphate moiety is attached.

57. Which of the following statements about E. coli RNA polymerase is correct? (A) The enzyme is composed of four subunits, namely αβρσ. (B) It requires the assistance of a DNA helicase to unwind double-stranded DNA for initiation of transcription. (C) The o subunit is responsible for recognizing the promoter sequence. (D) The o subunit is dissociated from the enzyme complex when the mRNA synthesis is complete. (E) The growth direction of the RNA chain is from 3' to 5'.

59. Which of the following statements about kcat is correct? (A) kcat is an indicator of the purity of an enzyme preparation. (B) kcat represents the substrate concentration required for an enzyme to reach50% of its maximum reaction velocity. (C) kcat reflects the half-life of an enzyme. (D) kcat represents the pH value at which the product dissociated from the enzyme. (E) kcat is defined as the number of substrates converted to products per enzyme molecule per unit time when the substrate concentration is saturated.

60. The structural and functional advantages of protein quaternary association do NOT include: (A) It can improve protein stability by reducing the combined molecular surface- to-volume ratio. (B) It can significantly enhance the efficiency of protein transportation. (C) The combination of different subunits can achieve synergy and function regulation. (D) Because same protein subunits can be assembled to form enzymes of different activities, genes can be used more efficiently. (E) Several functional groups required for the catalytic reaction can be brought together to form a functional enzyme catalytic center NTHU114

1. 下列選項中，  沒有  錯別字的是： (A) 在民眾缺乏警剔的前提下，要釐清假新聞的謬誤並不容易 (B) 陳醫師醫術精湛，又能視病如親，美好聲譽早已不徑而走 (C) 用極端負面的評論一筆抹殺別人功勞，既失公允又不厚道 (D) 聽聞親人驟逝的噩耗，他攤坐在地上痛哭，久久不能自已

2. 下列各組詞語，用字完全正確的選項是： (A) 浮雲蔽日／弊車羸馬／裨補闕漏 (B) 德懋棠蔭／圖文並茂／貌然行事 (C) 驀然回首／蠻陌之邦／默不關心 (D) 殫精竭慮／虎視耽耽／擔食壺漿

3. 下列選項「 」內之詞性，與其他三者  不同  的是： (A) 沙鷗翔集，「錦鱗」游泳 (B) 形骸且健，「方寸」甚安 (C) 吾愛孟夫子，「風流」天下聞 (D) 「漁樵」於江渚之上，侶魚蝦而友麋鹿

4. 下列各組「 」中的字詞，前後意思相同的是： (A) 「放」聲高歌／摩頂「放」踵 (B) 「比」肩而行／櫛次鱗「比」 (C) 英雄「蓋」世／冠「蓋」相望 (D) 左「顧」右盼／三「顧」茅廬