試題詳解

6. Which is not true for the following experimental techniques?
(A) DNase I footprinting assay is used to locate the specific protein-binding sites on DNA.
(B) In yeast, a temperature sensitive (ts) mutant can exhibit the mutant phenotype at a non-permissive temperature.
(C) Yeast one-hybrid analysis is useful for in vivo detection of physical interaction between two proteins.
(D) Gel mobility shift assay can be used for determining the ability of a protein to bind to a specific DNA fragment, and measuring the DNA binding affinity of the protein.
(E) The far-western blot analysis can be used to determine receptor-ligand interactions, and screen libraries of interacting proteins.