65. The rate of an enzyme catalyzed reaction:
1. can be determined by measuring the increase in product concentration with respect to time.
2. is usually dependent on temperature and pH.
3. at saturating substrate concentrations, becomes zero-order with respect to substrate.
4. is independent of the enzyme concentration.
(A) 1.
(B) 1 and 3.
(C) 2 and 4.
(D) 1, 2 and 3.
(E) All of them.